Journal of General Plant Pathology

, Volume 86, Issue 1, pp 55–59 | Cite as

Toothpick method to evaluate soil antagonism against the white root rot fungus, Rosellinia necatrix

  • Maho TakahashiEmail author
  • Hitoshi Nakamura
Disease Control


A method was developed to evaluate soil antagonism against the white root rot fungus, Rosellinia necatrix. Toothpicks colonized by the fungus were immersed into hot water to produce an extinction zone (EZ) of the fungus and then inserted into soil from different orchards. The EZ differed to various extents in unsterile soils; however, when soil was sterilized, the EZ disappeared. Using this method, we investigated the mechanism of the hot water drip irrigation method devised to control white root rot. The results showed the involvement of synergism between hot water and soil antagonists in the control measure against R. necatrix.


Hot water drip irrigation Rosellinia necatrix Soil antagonism Toothpick method 



We thank Dr. Naoyuki Matsumoto for critically reading the manuscript and helpful advice. This work was supported by a Grant from the Ministry of Agriculture, Forestry and Fisheries of Japan.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.


  1. Domsch KH, Gams W, Anderson TH (2007) Compendium of soil fungi. IHW-Verlag, EchingGoogle Scholar
  2. Eguchi N, Tokutake H, Yamagishi N (2008) Hot water treatment of Japanese pear trees is effective against white root rot caused by Rosellinia necatrix Prillieux. J Gen Plant Pathol 74:382–389CrossRefGoogle Scholar
  3. Eguchi N, Tokutake H, Tomita Y, Iwanami Y, Nakamura H (2009) Hot water treatment of Japanese pear trees is effective against white root rot (in Japanese). Plant Prot 63:127–130Google Scholar
  4. Freeman S, Sztejnberg A, Chet I (1986) Evaluation of Trichoderma as a biocontrol agent for Rosellinia necatrix. Plant Soil 94:163–170CrossRefGoogle Scholar
  5. Hermosa MR, Grondona I, Iturriaga EA, Diaz-Minguez JM, Castro C, Monte E, Garcia-Acha E (2000) Molecular characterization and identification of biocontrol isolates of Trichoderma spp. Appl Environ Microbiol 66:1890–1898CrossRefGoogle Scholar
  6. Kanadani G, Date H, Nasu H (1998) Effect of fluazinam soil-drench on white root rot of grapevine (in Japanese with English abstract). Jpn J Phytopathol 64:139–141CrossRefGoogle Scholar
  7. Köhl JJ, Molhoek WWML, Groenenboom-De Haas BBH, Goossen-Van De Geijn HHM (2009) Selection and orchard testing of antagonists suppressing conidial production by the apple scab pathogen Venturia inaequalis. Eur J Plant Pathol 123:401–414CrossRefGoogle Scholar
  8. López-Herrera CJ, Zea-Bonilla T (2007) Effects of benomyl, carbendazim, fluazinam and thiophanate methyl on white root rot of avocado. Crop Prot 26:1186–1192CrossRefGoogle Scholar
  9. Mendoza García RA, Martijn ten Hoopen G, Kass DCJ, Sánchez Garita VA, Krauss U (2003) Evaluation of mycoparasites as biocontrol agents of Rosellinia root rot in cocoa. Biol Control 27:210–227CrossRefGoogle Scholar
  10. Nakamura H (2013) Overview and prospects of hot water treatment against white root rot on fruit trees (in Japanese). Plant Prot 67:463–467Google Scholar
  11. Nakamura H, Uetake Y, Arakawa M, Okabe I, Matsumoto N (2000) Observations on the teleomorph of the white root rot fungus, Rosellinia necatrix, and a related fungus, Rosellinia aquila. Mycoscience 41:503–507CrossRefGoogle Scholar
  12. Pliego C, López-Herrera C, Ramos C, Cazora FM (2012) Developing tools to unravel the biological secrets of Rosellinia necatrix, an emergent threat to woody crops. Mol Plant Pathol 13:226–239CrossRefGoogle Scholar
  13. Ruano-Rosa D, López Herrera CJ (2009) Evaluation of Trichoderma spp. as biocontrol agents against avocado white root rot. Biol Control 51:66–71CrossRefGoogle Scholar
  14. Ruano-Rosa D, Cazorla FM, Bonilla N, Martín-Pérez R, De Vicente A, López-Herrera CJ (2014) Biological control of avocado white root rot with combined applications of Trichoderma spp. and rhizobacteria. Eur J Plant Pathol 138:751–762CrossRefGoogle Scholar
  15. Schena L, Nigro F, Ippolito A (2008) Integrated management of Rosellinia necatrix root rot on fruit tree crops. In: Ciancio A, Mukerji K (eds) Integrated management of plant pests and diseases. Integrated management of diseases caused by fungi, phytoplasma and bacteria, vol 3. Springer, Dordrecht, pp 137–158CrossRefGoogle Scholar
  16. Ten Hoopen GM, Krauss U (2006) Biology and control of Rosellinia bunodes, Rosellinia necatrix and Rosellinia pepo: a review. Crop Prot 25:89–107CrossRefGoogle Scholar
  17. Torres DE, Rojas-Martínez RI, Zavaleta-Mejía E, Guevara-Fefer P, Márquez-Guzmán GJ, Pérez-Martínez C (2017) Cladosporium cladosporioides and Cladosporium pseudocladosporioides as potential new fungal antagonists of Puccinia horiana Henn., the causal agent of chrysanthemum white rust. PLoS ONE 12:e0170782CrossRefGoogle Scholar
  18. Watanabe T (2002) Pictorial atlas of soil and seed fungi: morphologies of cultured fungi and key to species, 2nd edn. CRC Press, Boca RatonCrossRefGoogle Scholar

Copyright information

© The Phytopathological Society of Japan and Springer Japan KK, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Chiba Prefectural Agriculture and Forestry Research CenterChibaJapan
  2. 2.Institute of Fruit Tree and Tea ScienceNational Agriculture and Food Research OrganizationTsukubaJapan

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