Detection and molecular characterization of a phytoplasma in Eclipta prostrata in China
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Witches’ broom and phyllody, associated with phytoplasma infection, were observed on Eclipta prostrata, a common herb throughout the world, in Haikou, Hainan Province, China. Phytoplasma infection was confirmed using polymerase chain reaction and phytoplasma-specific primer pairs P1/P7, R16F2n/R16R2, rp(II)F/rp(II)R and subsequent amplicon sequencing. Based on the partial sequence of 16S rRNA gene and ribosomal protein gene, phylogenetic tree analysis and RFLP analysis of phytoplasma showed that the phytoplasma was a strain related to ‘Candidatus Phytoplasma aurantifolia’. To our knowledge, this is the first report of a phytoplasma in E. prostrata worldwide.
KeywordsPhytoplasma Eclipta prostrata Candidatus Phytoplasma aurantifolia
This research was support by the National Key Research and Development Program of China (Award No. 2018YFD0200900), Natural Science Foundation of Hubei Province (Award No. 2018CFB255).
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Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
- Li Y, Tian G, Xu Q, Piao C, Wang L, Guo M (2009) Molecular identification of cleome witches’-broom phytoplasma. Acta Phytopathol Sin 39:377–384Google Scholar
- Li Y, Xu Q, Tian G, Guo M, Piao C (2010) Molecular identification of crotalaria witches’-broom phytoplasma. Sci Silvae Sin 46:163–168Google Scholar
- Li Y, Tian G, Piao C, Guo M, Lin L (2011a) The molecular identification and phylogeny of Tephrosia witches’-broom phytoplasma. Forest Res 5:549–553Google Scholar