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Journal of General Plant Pathology

, Volume 86, Issue 1, pp 60–64 | Cite as

Detection and molecular characterization of a phytoplasma in Eclipta prostrata in China

  • Wang Chen
  • Yan Li
  • Xiaoping FangEmail author
Disease Note
  • 477 Downloads

Abstract

Witches’ broom and phyllody, associated with phytoplasma infection, were observed on Eclipta prostrata, a common herb throughout the world, in Haikou, Hainan Province, China. Phytoplasma infection was confirmed using polymerase chain reaction and phytoplasma-specific primer pairs P1/P7, R16F2n/R16R2, rp(II)F/rp(II)R and subsequent amplicon sequencing. Based on the partial sequence of 16S rRNA gene and ribosomal protein gene, phylogenetic tree analysis and RFLP analysis of phytoplasma showed that the phytoplasma was a strain related to ‘Candidatus Phytoplasma aurantifolia’. To our knowledge, this is the first report of a phytoplasma in E. prostrata worldwide.

Keywords

Phytoplasma Eclipta prostrata Candidatus Phytoplasma aurantifolia 

Notes

Acknowledgements

This research was support by the National Key Research and Development Program of China (Award No. 2018YFD0200900), Natural Science Foundation of Hubei Province (Award No. 2018CFB255).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

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Copyright information

© The Phytopathological Society of Japan and Springer Japan KK, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Key Laboratory of Biology and Genetics Improvement of Oil Crops, Ministry of Agriculture and Rural AffairsOil Crops Research Institute of Chinese Academy of Agricultural SciencesWuhanChina

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