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Journal of General Plant Pathology

, Volume 85, Issue 6, pp 413–423 | Cite as

Pseudomonas grimontii, causal agent of turnip bacterial rot disease in Japan

  • Hiroyuki SawadaEmail author
  • Harukuni Horita
  • Tomoo Misawa
  • Yuichi Takikawa
Bacterial and Phytoplasma Diseases

Abstract

From April to May 2012, a rot disease on turnips grown in greenhouses in southern Hokkaido, Japan caused severe leaf blight, brown discoloration of crown surface, and browning and rot of the interior of main roots. From diseased tissues, bacteria that formed pale beige- to cream-colored colonies were isolated and demonstrated to be pathogenic on turnip after inoculation and reisolation of the isolates. They were gram-negative, aerobic rods with one to three polar flagella, and identified as Pseudomonas grimontii based on biochemical and physiological characterizations, multilocus sequence analysis using concatenated sequences of 16S rRNA, rpoD, gyrB, and rpoB genes, and MALDI-TOF MS analysis using the Bruker MALDI Biotyper system. To the best of our knowledge, P. grimontii has not been isolated previously in Japan, so this study might be the first report of the species in Japan. Although the significance of P. grimontii as a plant pathogenic bacterium has not yet been fully evaluated, we proved in this study that the bacterium is the causal agent of the disease and that its virulence is strong. These findings indicate that it is necessary to record P. grimontii as a novel pathogen posing a threat to plants and requiring vigilance.

Keywords

Pseudomonas grimontii Brassica rapa var. rapa Bacterial rot MLSA MALDI Biotyper LOPAT 

Notes

Acknowledgements

We deeply appreciate Ms. C. Aoyagi and Ms. H. Nakajima (Genetic Resources Center, NARO) for their daily technical assistance. We also thank Mr. K. Takada (Central Agricultural Experiment Station, Hokkaido Research Organization), Ms. S. Yuuki (Hokkaido Prefectural Sorachi Agricultural Extension Center) and Mr. A. Hagiwara (Hokkaido College of Agriculture) for help with this work.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Human and animal rights

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

10327_2019_869_MOESM1_ESM.docx (42.5 mb)
Supplementary file1 (DOCX 43515 kb)

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Copyright information

© The Phytopathological Society of Japan and Springer Japan KK, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Genetic Resources CenterNational Agriculture and Food Research Organization (NARO)TsukubaJapan
  2. 2.Central Agricultural Experiment StationHokkaido Research OrganizationNaganumaJapan
  3. 3.Donan Agricultural Experiment StationHokkaido Research OrganizationHokutoJapan
  4. 4.Graduate School of Science and TechnologyShizuoka UniversityShizuokaJapan

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