Transcriptional analysis of defense-related genes induced by infection with the causal agent of downy mildew, Plasmopara viticola, in grapevine cultivar Shine Muscat
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Downy mildew caused by Plasmopara viticola is a devastating disease of grapevines. Grape cultivar Shine Muscat, bred in Japan, is moderately resistant to downy mildew in Japanese vineyards and produces grapes with good flavor. In infection assays, sporangia production on leaves inoculated with P. viticola was repressed in Shine Muscat and resistant cultivar Kober 5BB in comparison with susceptible culivar Katta Kurgan. Transcriptional analyses of these cultivars after inoculation with P. viticola revealed that the defense-related gene PR-2 was induced in Shine Muscat and Kober 5BB but not Katta Kurgan. These results demonstrate that Shine Muscat has a defense mechanism(s) that includes induction of defense-related genes such as PR-2. Analysis of the breeding line of Shine Muscat suggests that the mechanism(s) of disease resistance was transferred from Stueben to Shine Muscat.
KeywordsDefense-related gene Downy mildew Grapevine Plasmopara viticola Transcriptional analysis
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Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
- Ebrahim S, Usha K, Singh B (2011) Pathogenesis related (PR) proteins in plant defense mechanism. In: Méndez-Vilas A (ed) Science against microbial pathogens: communicating current research and technological advances. Formatex Research Center, Badajoz, pp 1043–1054Google Scholar
- Gessler C, Pertot I, Perazzolli M (2011) Plasmopara viticola: a review of knowledge on downy mildew of grapevine and effective disease management. Phytopathol Mediterr 50:3–44Google Scholar
- Kishi K (ed) (1998) Plant diseases in Japan. Zenkoku Noson Kyoiku Kyokai, Tokyo, pp 853 (in Japanese)Google Scholar
- Kono A, Ban Y, Sato A, Mitani N (2014) Evaluation of 17 table grape accessions for foliar resistance to downy mildew. In: Proceedings of the XIth International Conference on Grapevine Breeding and Genetics, Beijing, China. International Society for Horticultural Science, Leuven, Belgium, pp 207–209Google Scholar
- Malacarne G, Vrhovsek U, Zulini L, Cestaro A, Stefanini M, Mattivi F, Delledonne M, Velasco R, Moser C (2011) Resistance to Plasmopara viticola in a grapevine segregating population is associated with stilbenoid accumulation and with specific host transcriptional responses. BMC Plant Biol 11:114CrossRefPubMedPubMedCentralGoogle Scholar
- Polesani M, Bortesi L, Ferrarini A, Zamboni A, Fasoli M, Zadra C, Lovato A, Pezzotti M, Delledonne M, Polverari A (2010) General and species-specific transcriptional responses to downy mildew infection in a susceptible (Vitis vinifera) and a resistant (V. riparia) grapevine species. BMC Genom 11:117CrossRefGoogle Scholar
- Staudt G, Kassemeyer HH (1995) Evaluation of downy mildew resistance in various accessions of wild Vitis species. Vitis 34:225–228Google Scholar
- Yamada M, Yamane H, Sato A, Hirakawa N, Iwanami H, Yoshinaga K, Ozawa T, Mitani N, Shiraishi M, Yoshioka M, Nakajima I, Nakano M, Nakaune R (2008) New grape cultivar ‘Shine Muscat’ (in Japanese with English summary). Bull Natl Fruit Tree Res Stn 7:21–38Google Scholar