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Journal of General Plant Pathology

, Volume 85, Issue 1, pp 49–56 | Cite as

First report on DMI fungicide resistance in Gymnosporangium asiaticum, the causal agent of Japanese pear rust, in Japan

  • Kenji KikuharaEmail author
  • Kazuhiro Iiyama
  • Masaru Matsumoto
  • Naruto Furuya
Disease Control
  • 98 Downloads

Abstract

Japanese pear rust, caused by Gymnosporangium asiaticum Miyabe ex Yamada, is one of the most important fungal plant diseases affecting Japanese pear cultivation. Although sterol demethylation inhibitor (DMI) fungicides have controlled this disease effectively, outbreaks have been frequent in some areas of Japan since 2008. We, therefore, evaluated fungal populations from Fukuoka Prefecture for DMI fungicide sensitivity in vivo. Aeciospores on diseased pear leaves were collected in 2013 from four orchards in Fukuoka Prefecture and one control orchard in Ibaraki Prefecture. Juniperus chinensis var. kaizuka Hort. test plants were sprayed with difenoconazole and fenarimol at recommended rates, and the plants were inoculated with aeciospores. Difenoconazole and fenarimol effectively controlled the population of rust fungus from Ibaraki Prefecture. In contrast, fenarimol and difenoconazole were found to be ineffective against fungal populations from Fukuoka Prefecture. Subsequently, telia were collected from diseased juniper twigs in five areas in Fukuoka Prefecture and one control area in Ibaraki Prefecture in 2013 and 2014. Similarly, test plants of Japanese pear ‘Kousui’ were treated with difenoconazole or fenarimol and inoculated with basidiospores taken from those telia. Both difenoconazole and fenarimol were largely ineffective against the populations from Fukuoka Prefecture. In addition, field trials conducted in an experimental orchard in 2014 and 2015 showed that difenoconazole and fenarimol were not effective in controlling this disease. These results indicate the occurrence of Japanese pear rust resistant to DMI fungicides in Fukuoka Prefecture.

Keywords

Bioassay DMI fungicides Fungicide resistance Gymnosporangium asiaticum Japanese pear rust 

Notes

Acknowledgements

We thank Professor Hideo Ishii, Kibi International University, for providing diseased pear leaves and Mr. Takashi Nanba, JPPA, for providing diseased ‘Kaizuka’ twigs. Part of this work involved the study of agricultural chemicals contracted by JA Zen-Noh (National Federation of Agricultural Cooperative Associations).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

References

  1. Abe K, Kotobuki K (1998) Inheritance of high resistance to Venturia nashicola Tanaka et Yamamoto in Japanese pear (Pyrus pyrifolia Nakai) and Chinese pear (P. ussuriensis Maxim.). J Japan Soc Hort Sci 65:677–680CrossRefGoogle Scholar
  2. Akahira T (2017) Occurrence and disease control of DMI fungicide-resistant strains of Venturia inaequalis (apple scab fungus) in Aomori Prefecture (in Japanese with English summary). In: Inada M (ed) Abstracts of the 27th symposium of research committee on fungicide resistance, Morioka, Japan, Phytopathological Society of Japan, pp 46–54Google Scholar
  3. Brent KJ, Hollomon DW (2007) Fungicide resistance in crop pathogens: How can it be managed? FRAC monograph no. 1, 2nd revised edn. Fungicide Resistance Action Committee, Brussels, Belgium. http://www.frac.info/docs/default-source/publications/monographs/monograph-1.pdf. Accessed 18 Nov 2017
  4. Cools HJ, Hawkins NJ, Fraaije BA (2013) Constraints on the evolution of azole resistance in plant pathogenic fungi. Plant Pathol 62:36–42CrossRefGoogle Scholar
  5. de Waard MA, Andrade AC, Hayashi K, Schoonbeek HJ, Stergiopoulos I, Zwiers LH (2006) Impact of fungal drug transporters on fungicide sensitivity, multidrug resistance and virulence. Pest Manag Sci 62:195–207CrossRefGoogle Scholar
  6. Délye C, Bousset L, Corio-Costet MF (1998) PCR cloning and detection of point mutations in the eburicol 14α-demethylase (CYP51) gene from Erysiphe graminis f. sp. hordei, a “recalcitrant” fungus. Curr Genet 34:399–403CrossRefGoogle Scholar
  7. FRAC (Fungicide Resistance Action Committee) (2013) Pathogen risk list 2014. http://www.frac.info/docs/default-source/publications/pathogen-risk/pathogen-risk-list.pdf. Accessed 2017
  8. Godoy CV, Seixas CDS, Soares RM, Marcelino-Guimarães FC, Meyer MC, Costamilan LM (2016) Asian soybean rust in Brazil: past, present, and future. Pesqui Agropec Bras 51:407–421CrossRefGoogle Scholar
  9. Ishii H, Tanaka S (2016) The new pear cultivars ‘Akihime’ and ‘Risui’ are highly resistant to both scab and black spot diseases (abstract in Japanese). Jpn J Phytopathol 82:59 Google Scholar
  10. JPPA (Japan Plant Protection Association) (2018) Method of field tests for fungicide efficacy (deciduous fruit trees) (in Japanese author’s translation). http://www.jppa.or.jp/test/data/rakukin.pdf. Accessed 23 Jul 2018
  11. Kikuhara K (2016) Occurrence and management of DMI-fungicide-resistance in Venturia nashicola (scab) and Gymnosporangium asiaticum (rust) on Japanese pear in Fukuoka Prefecture (in Japanese with English summary). In: Inada M (ed) Abstracts of the 26th symposium of research committee on fungicide resistance, Okayama, Japan, Phytopathological Society of Japan, pp 64–74Google Scholar
  12. Kikuhara K, Ishii H (2008) Fenarimol resistance in Venturia nashicola, the scab fungus of Japanese pear in Fukuoka Prefecture (in Japanese with English summary). Kyushu Pl Prot Res 54:24–29 CrossRefGoogle Scholar
  13. Kikuhara K, Hashimoto H, Iiyama K, Matsumoto M, Furuya N (2018) The relationship between occurrence of Japanese pear rust and decreased efficacy of DMI fungicides: a retrospective cohort study in Fukuoka Prefecture (in Japanese with English summary). Jpn J Phytopathol 84:98–104CrossRefGoogle Scholar
  14. Kitajima H (1989) Detailed explanations of fruit diseases (in Japanese, author’s translation). Yokendo, Tokyo, pp 240–251 Google Scholar
  15. Kolmer JA, Ordonez ME, Groth JV (2009) The rust fungi. In: Encyclopaedia of life sciences (ELS). Wiley, Chichester, pp 1–8.  https://doi.org/10.1002/9780470015902.a0021264 Google Scholar
  16. Kudo A (1995) Gymnosporangium asiaticum (in Japanese). In: Ohata K, Araki T, Kiso A, Kudo A, Takahashi H (eds) Methods for isolation, cultivation, inoculation of plant pathogens. Japan Plant Protection Association, Tokyo, pp 235–236 Google Scholar
  17. Ma Z, Proffer TJ, Jacobs JL, Sundin GW (2006) Overexpression of the 14α-demethylase target gene (CYP51) mediates fungicide resistance in Blumeriella jaapii. Appl Environ Microbiol 72:2581–2585CrossRefGoogle Scholar
  18. MAFF [Ministry of Agriculture, Forestry and Fisheries] (2016) Japanese pear and grape acreage, yield and shipment volume in 2015 (in Japanese, author’s translation). http://www.maff.go.jp/j/tokei/kouhyou/sakumotu/sakkyou_kazyu/attach/pdf/index-5.pdf. Accessed 29 Nov 2017
  19. Nakamura K (2013) Pleasant epidemiology learning from basics (in Japanese author’s translation), 3rd edn. Igaku-shoin, Tokyo, pp 118–127Google Scholar
  20. Noguchi Y (1983) The control effects of systemic fungicides on Japanese pear rust (in Japanese with English summary). Bull Fukuoka Agric Res Cent B -2:71–77Google Scholar
  21. Oliver RP (2014) A reassessment of the risk of rust fungi developing resistance to fungicides. Pest Manag Sci 70:1641–1645CrossRefGoogle Scholar
  22. Schnabel G, Jones AL (2001) The 14α-demethylase (CYP51A1) gene is overexpressed in Venturia inaequalis strains resistant to myclobutanil. Phytopathology 91:102–110CrossRefGoogle Scholar
  23. Shibata T, Kagawa H, Ohtani T, Umemoto S (2012) Evaluation of susceptibility of 61 cultivars among 10 Juniperus species as telial hosts to Gymnosporangium asiaticum (in Japanese with English summary). CAFRC Res Bull 4:51–55Google Scholar
  24. Umemoto S, Murata A, Nagai Y (1989) Distance of sporidial dispersal of Japanese pear rust fungus, Gymnosporangium asiaticum Miyabe ex Yamada (in Japanese with English summary). Ann Phytopath Soc Japan 55:250–253CrossRefGoogle Scholar
  25. Watson PF, Rose ME, Ellis SW, England H, Kelly SL (1989) Defective sterol C5-6 desaturation and azole resistance: a new hypothesis for the mode of action of azole antifungals. Biochem Biophys Res Commun 164:1170–1175CrossRefGoogle Scholar
  26. Yamaguchi J, Inada M, Matsuzaki M (2000) Occurrence of DMI-fungicide-resistant isolates of Mycovellosiella nattrassii Deighton, causal fungus of leaf mold of eggplant (in Japanese with English summary). Jpn J Phytopathol 66:78–84CrossRefGoogle Scholar

Copyright information

© The Phytopathological Society of Japan and Springer Japan KK, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Fukuoka Agriculture and Forestry Research CenterFukuokaJapan
  2. 2.Faculty of AgricultureKyushu UniversityFukuokaJapan
  3. 3.Institute of Tropical AgricultureKyushu UniversityFukuokaJapan

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