Molecular characterization of Ralstonia solanacearum strains causing bacterial wilt of solanaceous crops in Myanmar by rep-PCR analysis
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Seventy strains of Ralstonia solanacearum from solanaceous crops in Myanmar were molecularly characterized by rep-PCR DNA fingerprinting. Cluster analysis based on the fingerprints (total 33 fingerprint types) revealed seven main clusters at 80% similarity level. Strains in phylotype I showed 29 fingerprint types and were divided into six clusters, most of which were closely correlated with host plant, geographic origin, sequevar and/or biovar. In contrast, phylotype II strains showed four DNA fingerprint types within a single cluster. These results suggest that genetic diversity of the strains is endemic and that phylotype I and II may be invasive strains.
KeywordsRalstonia solanacearum Rep-PCR Myanmar Cluster
We are grateful to the Ministry of Education, Culture, Sports, Science and Technology of Japan. We also thank Dr. Satoshi Kamitani, Bioresource and Bioenvironmental Sciences, Kyushu University for his support for data analysis. We deeply acknowledge Dr. Harry Evans, emeritus fellow for his English language editing. Part of this research was supported by the grants from the Project of the NARO Bio-Oriented Technology Research Advancement Institution (Research Program on Development of Innovative Technology No. 29014C).
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Conflict of interest
The authors declare that they have no conflict of interest.
Ethical approval/human and animal rights statement
This article does not contain any studies with human participants or animals performed by any of the authors.
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