Journal of General Plant Pathology

, Volume 84, Issue 3, pp 237–242 | Cite as

Disease suppressive activity of extracts from entomopathogenic fungus Ophiocordyceps sobolifera against chili anthracnose fungi Colletotrichum spp. in a pot experiment

  • Piyanoot Jaihan
  • Kusavadee Sangdee
  • Aphidech Sangdee
Disease Control

Abstract

This study evaluated the efficacy of the extracts of Ophiocordyceps sobolifera isolate Cod-NB1302 for the biological control of chili anthracnose disease caused by Colletotrichum capsici and C. gloeosporioides under pot conditions. Among the extracts, mycelial extract treatments provide the best reduction in disease severity. Interestingly, two bioactive constituents, adenosine and cordytropolone, from the mycelial extract, inhibited growth of the fungal pathogens. Moreover, these bioactive compounds had a synergistic effect against the fungal pathogens in a pot experiment. These results confirmed the disease suppressive activity of the mycelial extract.

Keywords

Anthracnose disease Antifungal activity Entomopathogenic fungi Ophiocordyceps sobolifera 

Notes

Acknowledgements

The authors acknowledge the Department of Biology, Faculty of Science, Mahasarakham University for support and equipment for this research. P. Jaihan gratefully thanks the Human Resource Development in Science Project (Science Achievement Scholarship of Thailand; SAST). Dr. Jolyon Dodgson, Faculty of Science, Mahasarakham University, is appreciated for language editing of the manuscript.

Compliance with ethical standards

Conflict of interest

The authors confirm that there are no conflicts of interest regarding any experimental data.

References

  1. Anand T, Bhaskaran R (2009) Exploitation of plant products and bioagents for ecofriendly management of chilli fruit rot disease. J Plant Prot Res 49:195–203CrossRefGoogle Scholar
  2. Eghdami A, Sadeghi F (2010) Determination of total phenolic and flavonoids contents in methanolic and aqueous extract of Achillea Millefolium. Org Chem J 2:81–84Google Scholar
  3. Fallik E, Grinberg S (1992) Hinokitiol: a natural substance that controls postharvest diseases in eggplant and pepper fruits. Postharvest Biol Technol 2:137–144CrossRefGoogle Scholar
  4. Fendler E, Groziak P (2002) Efficacy of alcohol-based hand sanitizers against fungi and viruses. Infect Control Hosp Epidemiol 23:61–62CrossRefPubMedGoogle Scholar
  5. Hartman GL, Wang TC (1992) Characteristics of two Colletotrichum species and evaluation of resistance to anthracnose in pepper. In: Proceedings of third international conference on plant protection in the tropics, vol 6. Malaysian Plant Protection Society, Kuala Lumpur, pp 202–205Google Scholar
  6. Hasyim A, Setiawati W, Sutarya R (2014) Screening for resistance to anthracnose caused by Colletotrichum acutatum in chili pepper (Capsicum annuum L.) in Kediri, East Java. Adv Agric Bot 6:104–118Google Scholar
  7. Huang L, Li Q, Chen Y, Wang X, Zhou X (2009) Determination and analysis of cordycepin and adenosine in the products of Cordyceps spp. Afr J Microbiol Res 3:957–961Google Scholar
  8. Imtiaj A, Lee TS (2007) Screening of antibacterial and antifungal activities from Korean wild mushrooms. World J Agric Sci 3:316–321Google Scholar
  9. Jaihan P, Sangdee K, Sangdee A (2016) Selection of entomopathogenic fungus for biological control of chili anthracnose disease caused by Colletotrichum spp. Eur J Plant Pathol 146:551–564CrossRefGoogle Scholar
  10. Lee EJ, Ahn YJ, Lee HS, Chung N (2012) Biocontrol of pepper anthracnose by a new Streptomyces sp. A1022 under greenhouse condition. J Korean Soc Appl Biol Chem 55:447–449CrossRefGoogle Scholar
  11. McDonnell G, Russell AD (1999) Antiseptics and disinfectants: activity, action, and resistance. Clin Microbiol Rev 12:147–179PubMedPubMedCentralGoogle Scholar
  12. Morita Y, Matsumura E, Okabe T, Shibata M, Sugiura M, Ohe T, Tsujibo H, Ishida N, Inamori Y (2003) Biological activity of tropolone. Biol Pharm Bull 26:1487–1490CrossRefPubMedGoogle Scholar
  13. Oanh LTK, Korpraditskul V, Rattanakreetakul C (2004) A pathogenicity of anthracnose fungus, Colletotrichum capsici on various Thai chilli varieties. Kasetsart J (Nat Sci) 38:103–108Google Scholar
  14. Pakdeevaraporn P, Wasee S, Taylor PWJ, Mongkolporn O (2005) Inheritance of resistance to anthracnose caused by Colletotrichum capsici in Capsicum. Plant Breed 124:206–208CrossRefGoogle Scholar
  15. Peng XX, Chai YQ, Zhu BC, Jin YW, Li XL, Yu LS (2015) The protective effects of N6-(2-hydroxyethyl)-adenosine extracted from Ophiocordyceps sobolifera on renal ischemia reperfusion injury (IRI) in mice (in Chinese with English abstract). Mycosystema 34:311–320Google Scholar
  16. Peres NAR, Souza NL, Peever TL, Timmer LW (2004) Benomyl sensitivity of isolates of Colletotrichum acutatum and C. gloeosporiodes from citrus. Plant Dis 88:125–130CrossRefGoogle Scholar
  17. Poonpolgul S, Kumphai S (2007) Chilli pepper anthracnose in Thailand. Country report. In: Oh DG, Kim KT (eds) Abstracts of the first international symposium on chilli anthracnose. National Horticultural Research Institute, Rural Development of Administration, Republic of Korea, p 23Google Scholar
  18. Sangdee K, Nakbanpote W, Sangdee A (2015) Isolation of the entomopathogenic fungal strain Cod-MK1201 from a cicada nymph and assessment of its antibacterial activities. Int J Med Mushrooms 17:51–63CrossRefPubMedGoogle Scholar
  19. Saniewski M, Saniewska A, Kanlayanarat S (2007) Biological activities of tropolone and hinokitiol: the tools in plant physiology and their practical use. Acta Hortic 755:133–142CrossRefGoogle Scholar
  20. Seephonkai P, Isaka M, Kittakoop P, Trakulnaleamsai S, Rattanajak R, Tanticharoen M, Thebtaranonth Y (2001) A new tropolone from the insect pathogenic fungus Cordyceps sp. BCC 1681. J Antibiot 54:751–752CrossRefPubMedGoogle Scholar
  21. Sugar AM, McCaffrey RP (1998) Antifungal activity of 3′-deoxyadenosine (cordycepin). Antimicrob Agents Chemother 42:1424–1427PubMedPubMedCentralGoogle Scholar
  22. Than PP, Jeewon R, Hyde KD, Pongsupasamit S, Mongkolporn O, Taylor PWJ (2008a) Characterization and pathogenicity of Colletotrichum species associated with anthracnose on chilli (Capsicum spp.) in Thailand. Plant Pathol 57:562–572CrossRefGoogle Scholar
  23. Than PP, Prihastuti H, Phoulivong S, Taylor PWJ, Hyde KD (2008b) Chilli anthracnose disease caused by Colletotrichum species. J Zhejiang Univ Sci B 9:764–778CrossRefPubMedPubMedCentralGoogle Scholar
  24. Varughese T, Riosa N, Higginbotham S, Arnold AE, Coley PD, Kursar TA, Gerwick WH, Rios LC (2012) Antifungal depsidone metabolites from Cordyceps dipterigena, an endophytic fungus antagonistic to the phytopathogen Gibberella fujikuroi. Tetrahedron Lett 53:1624–1626CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© The Phytopathological Society of Japan and Springer Japan KK, part of Springer Nature 2018

Authors and Affiliations

  • Piyanoot Jaihan
    • 1
  • Kusavadee Sangdee
    • 2
  • Aphidech Sangdee
    • 1
    • 3
  1. 1.Department of Biology, Faculty of ScienceMahasarakham UniversityMaha SarakhamThailand
  2. 2.Microbiology Group, Biomedical Sciences Research Unit, Faculty of MedicineMahasarakham UniversityMaha SarakhamThailand
  3. 3.Microbiology and Applied Microbiology Research Unit, Faculty of ScienceMahasarakham UniversityMaha SarakhamThailand

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