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Control of Fusarium wilt of melon by combined treatment with biocontrol, plant-activating, and soil-alkalizing agents

  • Iori ImazakiEmail author
  • Ikuo Kadota
Disease Control
  • 65 Downloads

Abstract

Fusarium wilt of melon is one of the destructive diseases of melon worldwide. To develop a method to control the soilborne disease Fusarium wilt of melon, we applied various combinations of a crop-protective microbe, a plant activator, and a soil pH increaser during seedling cultivation in preliminary greenhouse tests and chose the crop-protective Fusarium oxysporum strain SL0037, plant activator probenazole (PBZ), and soil-alkalizer slag fertilizer (SFR) for further tests in the field. From 2012 to 2014, the effects of treatment combinations with SL0037, PBZ, and SFR were tested in a field that was amended with the melon wilt pathogen F. oxysporum f. sp. melonis. Although disease incidence varied among the 3 years of the study, the main stem of melon plants was longer than that of the untreated control plants or those treated with one or two of the three agents. Results suggest a combination of SL0037, PBZ, and SFR treatments can be used to control Fusarium wilt of melon and improve growth of melon plants.

Keywords

Fusarium wilt of melon Fusarium oxysporum Probenazole Converter slag 

Notes

Acknowledgements

We are grateful to Drs. Yoshiro Mikoshiba, Seiji Kanematsu, Takashi Kobayashi, Ryo Yamamoto, Koya Sugawara, and Megumi Yoshida for valuable suggestions; Kazuhiro Hayasaka, Yoichi Kodate, and Masayuki Goto for technical assistance in the field experiments; and the members of the Department of Planning and General Administration for their cooperation.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

10327_2018_833_MOESM1_ESM.pdf (100 kb)
Fig. S1 Diagram of the field plots (PDF 99 KB)
10327_2018_833_MOESM2_ESM.pdf (163 kb)
Fig. S2 Effects of plant activator or mycelial extract treatments with Fusarium oxysporum strain SL0037 and soil alkalizer (slag fertilizer) on incidence of Fusarium wilt of melon in a greenhouse. Seeds of melon (cv. Kintaro) were sown in plug trays. Fifteen days after seeding, seedlings grown in plug trays were transplanted to plastic pots filled with soil infested with F. oxysporum f. sp. melonis strain Mel02010. Treatments: 1, untreated control; 2, SL0037; 3, SL0037 + SFR (slag fertilizer); 4, SL0037 + SFR + 192 mg a.i./l PBZ (probenazole) at seeding (treatment at seeding); 5, SL0037 + SFR + 96 mg a.i./l PBZ on day 11 (treatment 11 days after seeding); 6, SL0037 + SFR + 960 mg a.i./l PBZ on day 11; 7, SL0037 + SFR + 67.2 mg a.i./l TDL (tiadinil) at seeding; 8, SL0037 + SFR + 67.2 mg a.i./l TDL on day 11; 9, SL0037 + SFR + 672 mg a.i./l TDL on day 11; 10, SL0037 + SFR + 206 mg a.i./l VMA (validamycin A) at seeding; 11, SL0037 + SFR + 206 mg a.i./l VMA on day 11; 12, SL0037 + SFR + 2060 mg a.i./l VMA on day 11; 13, SL0037 + SFR + 20 mg a.i./l LEN (a fungal mycelial extract) at seeding; 14, SL0037 + SFR + 20 mg a.i./l LEN on day 11; treatment 14, SL0037 + SFR + 200 mg a.i./l LEN on day 11 (PDF 163 KB)
10327_2018_833_MOESM3_ESM.pdf (281 kb)
Fig. S3 Symptoms of Fusarium wilt of melon plants in field plots. (a) Leakage of droplets of gummy exudates, (b) blight of stem tip, (c) wilting (curing) at leaf margin, (d), growth inhibition, (e) yellowing, (f) systemic wilting, (g) and death (PDF 281 KB)
10327_2018_833_MOESM4_ESM.pdf (224 kb)
Fig. S4 Effect of Fusarium oxysporum strain SL0037 on plants other than melon. Photographs were taken at 16 (squash), 24 (spinach and cabbage), 26 (cucumber and tomato), 30 (lettuce), or 33 (eggplant and pepper) days after seeding and SL0037 inoculation (PDF 224 KB)
10327_2018_833_MOESM5_ESM.docx (53 kb)
Supplementary material 5 (DOCX 53 KB)

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Copyright information

© The Phytopathological Society of Japan and Springer Japan KK, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Tohoku Agricultural Research CenterNational Agriculture and Food Research OrganizationMoriokaJapan

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