Journal of General Plant Pathology

, Volume 84, Issue 1, pp 27–34 | Cite as

Decreased potassium fertilization is associated with increased pathogen growth and disease severity caused by Acidovorax citrulli in melon foliage

  • Naama Zimerman-Lax
  • Dafna Tamir-Ariel
  • Moshe Shenker
  • Saul BurdmanEmail author
Bacterial and Phytoplasma Diseases


The gram-negative bacterium Acidovorax citrulli causes bacterial fruit blotch (BFB) disease of cucurbits, which represents a serious threat to melon and watermelon production worldwide. To date, there are no efficient means to manage the disease, and reliable resistance sources for cucurbit germplasm are lacking. Mineral nutrition markedly affects plant diseases. Recently, we reported that disease severity on melon foliage and A. citrulli growth in the leaf tissue were significantly influenced by the form of nitrogen supply. In the present study, we investigated the influence of potassium nutrition on BFB severity and A. citrulli establishment in the foliage of melon plants. Fertilization with relatively low concentrations of potassium increased these variables compared with higher potassium concentrations. Since establishment of A. citrulli during the growing season is assumed to increase the incidence of fruit infection, the fact that mineral nutrition influences BFB incidence in the plant foliage is of particular importance.


Acidovorax citrulli Bacterial fruit blotch Cucumis melo Potassium Fertilization 



This work was funded by the Agriculture, Environment and Natural Resources Center of the Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem.


  1. Bahar O, Burdman S (2010) Bacterial fruit blotch: a threat to the cucurbit industry. Israel J Plant Sci 58:19–32CrossRefGoogle Scholar
  2. Bahar O, Efrat M, Hadar E, Dutta B, Walcott RR, Burdman S (2008) New subspecies-specific polymerase chain reaction-based assay for the detection of Acidovorax avenae subsp. citrulli. Plant Pathol 57:754–763CrossRefGoogle Scholar
  3. Bahar O, Kritzman G, Burdman S (2009) Bacterial fruit blotch of melon: screens for disease tolerance and role of seed transmission in pathogenicity. Eur J Plant Pathol 123:71–83CrossRefGoogle Scholar
  4. Burdman S, Walcott R (2012) Acidovorax citrulli: generating basic and applied knowledge to tackle a global threat to the cucurbit industry. Mol Plant Pathol 13:805–815CrossRefPubMedGoogle Scholar
  5. Burdman S, Kots N, Kritzman G, Kopelowitz J (2005) Molecular, physiological, and host-range characterization of Acidovorax avenae subsp. citrulli isolates from watermelon and melon in Israel. Plant Dis 89:1339–1347CrossRefGoogle Scholar
  6. Datnoff LE, Elmer WH, Huber DM (2007) Mineral nutrition and plant disease. APS Press, St. PaulGoogle Scholar
  7. Hopkins DL (1995) Copper-containing fungicides reduce the spread of bacterial fruit blotch of watermelon in the greenhouse. Phytopathology 85:510 (Abstract)Google Scholar
  8. Hopkins DL, Thompson CM (2002) Seed transmission of Acidovorax avenae subsp. citrulli in cucurbits. HortScience 37:924–926Google Scholar
  9. Hopkins DL, Cucuzza JD, Watterson JC (1996) Wet seed treatments for the control of bacterial fruit blotch of watermelon. Plant Dis 80:529–532CrossRefGoogle Scholar
  10. Huber DM, Haneklaus S (2007) Managing nutrition to control plant disease. Landbauforsch Volk 57:313–322Google Scholar
  11. Jones JJB, Case VW (1990) Sampling, handling, and analyzing plant tissue samples. In: Westman RL (ed) Soil testing and plant analysis, 3rd edn. Soil Science Society of America, Madison, pp 389–427Google Scholar
  12. Jones JB, Huber DM (2007) Magnesium and plant disease. In: Datnoff LE, Elmer WH, Huber DM (eds) Mineral nutrition and plant disease. APS Press, St. Paul, pp 95–100Google Scholar
  13. Latin RX, Hopkins DL (1995) Bacterial fruit blotch of watermelon: the hypothetical exam question becomes reality. Plant Dis 79:761–765CrossRefGoogle Scholar
  14. Maathuis FJM, Sanders D (1996) Mechanisms of potassium absorption by higher plant roots. Physiol Plant 96:158–168CrossRefGoogle Scholar
  15. Prabhu AS, Fageria NK, Huber DM, Rodrigues FA (2007) Potassium and plant disease. In: Datnoff LE, Elmer WH, Huber DM (eds) Mineral nutrition and plant disease. APS Press, St. Paul, pp 57–78Google Scholar
  16. Rahman M, Punja ZK (2007) Calcium and plant disease. In: Datnoff LE, Elmer WH, Huber DM (eds) Mineral nutrition and plant disease. APS Press, St. Paul, pp 79–93Google Scholar
  17. Rane KK, Latin RX (1992) Bacterial fruit blotch of watermelon: association of the pathogen with seed. Plant Dis 76:509–512CrossRefGoogle Scholar
  18. Reuter DJ, Robinson JB (1997) Plant analysis: an interpretation manual, 2nd edn. CSIRO Publishing, CollingwoodGoogle Scholar
  19. Schaad NW, Sowell G, Goth RW, Colwell RR, Webb RE (1978) Pseudomonas pseudoalcaligenes subsp. citrulli subsp. nov. Int J Syst Bacteriol 28:117–125CrossRefGoogle Scholar
  20. Schaad NW, Postnikova E, Randhawa PS (2003) Emergence of Acidovorax avenae subsp. citrulli as a crop threatening disease of watermelon and melon. In: Iacobellis NS, Collmer A, Hutcheson SW, Mansfield JW, Morris CE, Murillo J, Schaad NW, Stead DE, Surico G, Ullrich MS (eds) Pseudomonas syringae and related pathogens. Kluwer, Dordrecht, pp 573–581CrossRefGoogle Scholar
  21. Schaad NW, Postnikova E, Sechler A, Claflin LE, Vidaver AK, Jones JB, Agarkova I, Ignatov A, Dickstein E, Ramundo BA (2008) Reclassification of subspecies of Acidovorax avenae as A. avenae (Manns 1905) emend., A. cattleyae (Pavarino, 1911) comb. nov., A. citrulli (Schaad et al., 1978) comb. nov., and proposal of A. oryzae sp. nov. Syst Appl Microbiol 31:434–446CrossRefPubMedGoogle Scholar
  22. Somodi GC, Jones JB, Hopkins DL, Stall RE, Kucharek TA, Hodge NC, Watterson JC (1991) Occurrence of a bacterial watermelon fruit blotch in Florida. Plant Dis 75:1053–1056CrossRefGoogle Scholar
  23. Walcott RR, Fessehaie A, Castro AC (2004) Differences in pathogenicity between two genetically distinct groups of Acidovorax avenae subsp. citrulli on cucurbit hosts. J Phytopathol 152:277–285CrossRefGoogle Scholar
  24. Walcott RR, Castro AC, Fessehaie A, Ling K (2006) Progress towards a commercial PCR-based assay for Acidovorax avenae subsp. citrulli. Seed Sci Technol 34:101–116CrossRefGoogle Scholar
  25. Wall GC, Santos VM (1988) A new bacterial disease on watermelon in the Mariana Islands. Phytopathology 78:1605 (Abstract)Google Scholar
  26. Walter DR, Bingham IJ (2007) Influence of nutrition on disease development caused by fungal pathogens: implications for plant disease control. Ann Appl Biol 151:307–324CrossRefGoogle Scholar
  27. Wang M, Zheng Q, Shen Q, Guo S (2013) The critical role of potassium in plant stress response. Int J Mol Sci 14:7370–7390CrossRefPubMedPubMedCentralGoogle Scholar
  28. Willems A, Goor M, Thielemans S, Gillis M, Kersters K, De Ley J (1992) Transfer of several phytopathogenic Pseudomonas species to Acidovorax as Acidovorax avenae subsp. avenae subsp. nov., comb. nov., Acidovorax avenae subsp. citrulli. Acidovorax avenae subsp. cattleyae, and Acidovorax konjaci. Int J Syst Bacteriol 42:107–119CrossRefPubMedGoogle Scholar
  29. Zimerman-Lax N, Shenker M, Tamir-Ariel D, Perl-Treves R, Burdman S (2016) Effects of nitrogen nutrition on disease development caused by Acidovorax citrulli on melon foliage. Eur J Plant Pathol 145:123–137CrossRefGoogle Scholar

Copyright information

© The Phytopathological Society of Japan and Springer Japan KK 2017

Authors and Affiliations

  • Naama Zimerman-Lax
    • 1
  • Dafna Tamir-Ariel
    • 1
  • Moshe Shenker
    • 2
  • Saul Burdman
    • 1
    Email author
  1. 1.Department of Plant Pathology and Microbiology, The Robert H. Smith Faculty of Agriculture, Food and EnvironmentThe Hebrew University of JerusalemRehovotIsrael
  2. 2.Department of Soil and Water Sciences, The Robert H. Smith Faculty of Agriculture, Food and EnvironmentThe Hebrew University of JerusalemRehovotIsrael

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