Journal of General Plant Pathology

, Volume 83, Issue 5, pp 291–298 | Cite as

Phylogenetic relationship and fungicide sensitivity of members of the Colletotrichum gloeosporioides species complex from apple

  • Shiori YokosawaEmail author
  • Naoki Eguchi
  • Ken-ichi Kondo
  • Toyozo Sato
Fungal Diseases


The members of the Colletotrichum gloeosporioides species complex (CGSC), the dominant pathogens of apple bitter rot in Nagano prefecture, Japan, were reidentified and the relationship between the species and fungicide sensitivity was revealed. Based on phylogenetic analysis of the ApMat locus with the neighbor-joining (NJ) method, isolates from apple contained three species of the CGSC; C. fructicola, C. aenigma, C. siamense, and three clades of the CGSC: Clade V, S and K. Colletotrichum fructicola and Clade S dominated in Nagano Prefecture. Isolates of C. siamense, C. aenigma and Clade V, S and K remained sensitive to benomyl and quinone outside inhibitor (QoI) fungicides, while C. fructicola often developed resistance to benomyl and QoI fungicides. These results suggest that the development of fungicide resistance differs among members of the CGSC.


Colletotrichum gloeosporioides species complex Apple bitter rot Reidentification ApMat Fungicide sensitivity 



We thank Mr. Yasuhiko Iwanami and the Nagano Prefecture Agricultural Extension Center for help in material collections; Mr. Kazuya Minemura, Ms. Akiko Yokota and Ms. Mariko Katsuyama for technical assistance; and Mr. Shuichi Kato for helpful suggestions in this study.

Supplementary material

10327_2017_732_MOESM1_ESM.xlsx (14 kb)
Supplementary material 1 (XLSX 15 kb)


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Copyright information

© The Phytopathological Society of Japan and Springer Japan KK 2017

Authors and Affiliations

  1. 1.Nagano Prefecture Fruit Tree Experimental StationSuzakaJapan
  2. 2.Nagano Prefecture Agricultural Experiment & Extension DivisionNaganoJapan
  3. 3.Genetic Resources CenterNational Agriculture and Food Research OrganizationTsukubaJapan

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