Skip to main content
SpringerLink
Log in

First report of spinach leaf spot caused by Stemphylium sp. Subgroups C2 and E3 and pathogenicity comparison of the pathogen with related pathogens

  • Fungal Diseases
  • Published:
Journal of General Plant Pathology Aims and scope Submit manuscript

Abstract

Leaf spots on spinach were found in three greenhouses in Hokkaido and Iwate, Japan, in 2011–2012. Three isolates obtained from the lesions were classified into Stemphylium sp. Subgroups C2 and E3, based on morphology and molecular analyses. We compared the three isolates with two reference isolates belonging to Subgroup C2 from Welsh onion and leek in terms of pathogenicity. All five isolates caused leaf spot symptoms on spinach and leaf blight on Welsh onion in inoculation tests. This is the first report of spinach leaf spot caused by Stemphylium sp. Subgroups C2 and E3.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

References

  • Bassimba DDM, Mira JL, Vicent A (2014) First report of leaf spot of spinach caused by Pleospora betae in Spain. Plant Dis 98:1583

    Article  Google Scholar 

  • Câmara MPS, O’Neill NR, van Berkum P (2002) Phylogeny of Stemphylium spp. based on ITS and glyceraldehyde-3-phosphate dehydrogenase gene sequences. Mycologia 94:660–672

    Article  PubMed  Google Scholar 

  • Inderbitzin P, Mehta YR, Berbee ML (2009) Pleospora species with Stemphylium anamorphs: a four locus phylogeny resolves new lineages yet does not distinguish among species in the Pleospora herbarum clade. Mycologia 101:329–339

    Article  CAS  PubMed  Google Scholar 

  • Koike ST, Henderson DM, Butler EE (2001) Leaf spot disease of spinach in California caused by Stemphylium botryosum. Plant Dis 85:126–130

    Article  Google Scholar 

  • Kurose D, Misawa T, Suzui T, Ichikawa K, Kisaki G, Hoang LH, Furuya N, Tsuchiya K, Tsushima S, Sato T (2015) Taxonomic re-examination of several Japanese Stemphylium strains based on morphological and molecular phylogenetic analyses. J Gen Plant Pathol 81:358–367

    Article  Google Scholar 

  • Leach CM, Aragaki M (1970) Effects of temperature on conidium characteristics of Ulocladium chartarum and Stemphylium floridanum. Mycologia 62:1071–1076

    Article  Google Scholar 

  • Misawa T (2012) Studies on the epidemiology and control of Welsh onion leaf blight caused by Stemphylium vesicarium (in Japanese with English summary). Rep Hokkaido Pref Agric Exp Stn 132:1–90

    Google Scholar 

  • Misawa T, Yasuoka S (2012) The life cycle of Stemphylium vesicarium, the causal agent of Welsh onion leaf blight. J Gen Plant Pathol 78:18–29

    Article  Google Scholar 

  • Misawa T, Tomizawa Y, Ueno R, Sugawara A (2014) Leaf blight of leek caused by Stemphylium vesicarium (in Japanese with English summary). Annu Rep Plant Prot North Jpn 65:72–75

    Google Scholar 

  • Sakai H, Harasawa K, Urushibara T, Shiraishi T (2002) Leaf spot disease of spinach by Stemphylium botryosum (Abstract in Japanese). Jpn J Phytopathol 68:65

    Google Scholar 

Download references

Author information

Author notes

  1. Daisuke Kurose

    Present address: CABI Europe-UK, Egham, Surrey, TW20 9TY, UK

  2. Seiya Tsushima

    Present address: Faculty of Agriculture, Tokyo University of Agriculture, Atsugi, Kanagawa, 243−0034, Japan

Authors and Affiliations

  1. Donan Agricultural Experiment Station, Hokkaido Research Organization, Hokuto, Hokkaido, 041-1201, Japan

    Tomoo Misawa

  2. Natural Resources Inventory Center, National Institute for Agro-Environmental Sciences, Tsukuba, Ibaraki, 305-8604, Japan

    Daisuke Kurose & Seiya Tsushima

  3. Tokachi Agricultural Experiment Station, Hokkaido Research Organization, Memuro, Hokkaido, 082-0081, Japan

    Miyuki Kayamori

  4. Iwate Agricultural Research Center, Kitakami, Iwate, 024-0003, Japan

    Yasuya Iwadate

Authors
  1. Tomoo Misawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Daisuke Kurose
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Miyuki Kayamori
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yasuya Iwadate
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Seiya Tsushima
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Tomoo Misawa.

Additional information

The nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank databases as accessions LC175742–175744, 175746–175748, 175750–175752, 175754–175756.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Misawa, T., Kurose, D., Kayamori, M. et al. First report of spinach leaf spot caused by Stemphylium sp. Subgroups C2 and E3 and pathogenicity comparison of the pathogen with related pathogens. J Gen Plant Pathol 83, 147–151 (2017). https://doi.org/10.1007/s10327-017-0711-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10327-017-0711-1

Keywords

Search

Navigation