Journal of General Plant Pathology

, Volume 83, Issue 3, pp 121–130 | Cite as

First report of leaf sheath rot of Welsh onion caused by nine taxa of Rhizoctonia spp. and characteristics of the pathogens

  • Tomoo Misawa
  • Daisuke Kurose
  • Shiro Kuninaga
Fungal Diseases


From 2007 to 2013, a disease of Welsh onion, causing leaf sheath rot and concomitant death of outer leaves was found in 20 fields in Hokkaido, Japan. We obtained 20 Rhizoctonia isolates from diseased tissues and identified them based on the number of nuclei, hyphal fusion reactions, and molecular techniques using specific PCR primers and sequence of the rDNA-ITS region. The 20 isolates consisted of 16 multinucleate and four binucleate isolates. Of the multinucleate isolates, five were found to be so far unknown and designated here as Rhizoctonia solani AG-4 hybrid subgroup between HG-I and HG-II. Others were identified as AG-1 IB (three isolates), AG-2-2 IIIB (two isolates), AG-4 HG-I (two isolates), AG-1 IC (one isolate), AG-2-1 (one isolate), AG-4 HG-II (one isolate) and AG-5 (one isolate). All four binucleate isolates were binucleate Rhizoctonia AG-U. Original symptoms were reproduced on all plants inoculated with these isolates. Thus, we revealed that as many as nine taxa of Rhizoctonia spp. were associated with the disease. This is the first report of leaf sheath rot of Welsh onion caused by Rhizoctonia spp.


Allium fistulosum Hokkaido Hybrid Leaf sheath Subgroup Rhizoctonia 



This study was supported in part by Grants-in-Aid for Regional R&D Proposal-Based Program from Northern Advancement Center for Science & Technology of Hokkaido Japan. For help with this work, we thank staff members of Hokkaido Prefectural Oshima, Sorachi and Iburi Agricultural Extension Centers, Japan. We thank A. Sumino (Hokkaido Research Organization) for valuable comments and Dr. N. Matsumoto (Hokkaido University, Japan) for critically reading the manuscript and correcting the English.


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Copyright information

© The Phytopathological Society of Japan and Springer Japan 2017

Authors and Affiliations

  1. 1.Donan Agricultural Experiment StationHokkaido Research OrganizationHokutoJapan
  2. 2.CABI Europe-UKSurreyUK
  3. 3.Health Sciences University of HokkaidoIshikari-TobetsuJapan

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