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Journal of General Plant Pathology

, Volume 82, Issue 5, pp 268–272 | Cite as

Hammer blot-mediated RNA extraction: an inexpensive, labor-saving method to extract RNA for plant virus detection

  • Md. Shamim Akhter
  • Takashi Yaeno
  • Naoto Yamaoka
  • Masamichi Nishiguchi
  • Kappei KobayashiEmail author
Viral and Viroid Diseases

Abstract

Here we report a novel method to extract nucleic acids for virus detection. Plant tissue in a filter paper sandwich was hit with a hammer, and the crude sap was adsorbed by the filter paper, as in hammer blotting for tissue-print immunodetection of plant viruses. Nucleic acids were extracted from the paper with a guanidine-containing buffer and purified through standard extraction and precipitation. Both viral and cellular RNAs in the extracted RNA were detectable by RT-PCR. The blots can be stored at 4 °C for more than a few days to detect viral RNA, but not cellular RNA.

Keywords

Hammer blot Nucleic acid extraction Plant virus 

Notes

Acknowledgments

This study was supported in part by the United Graduate School of Agricultural Sciences, Ehime University, JSPS KAKENHI Grants Nos. 24658044 and 15K14664. MSA was supported by MEXT, Japan.

Supplementary material

10327_2016_673_MOESM1_ESM.pdf (3.5 mb)
Supplementary material 1 (PDF 3535 kb)

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Copyright information

© The Phytopathological Society of Japan and Springer Japan 2016

Authors and Affiliations

  • Md. Shamim Akhter
    • 1
  • Takashi Yaeno
    • 1
    • 2
  • Naoto Yamaoka
    • 1
    • 2
  • Masamichi Nishiguchi
    • 2
  • Kappei Kobayashi
    • 1
    • 2
    Email author
  1. 1.The United Graduate School of Agricultural SciencesEhime UniversityMatsuyamaJapan
  2. 2.Faculty of AgricultureEhime UniversityMatsuyamaJapan

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