Analysis of rice RNA-dependent RNA polymerase 6 (OsRDR6) gene in response to viral, bacterial and fungal pathogens
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RNA-dependent RNA polymerases (RDRs) play key roles in gene silencing. The rice RDR6 gene was analyzed in response to viral, bacterial and fungal pathogens, after inoculation of a rice mutant line of OsRDR6, shl2-rol, with Cucumber mosaic virus, Rice necrosis mosaic virus, Xanthomonas oryzae pv. oryzae or Magnaporthe oryzae. Compared with the wild type, the mutant line accumulated more viral RNA after inoculation with the viruses and developed more severe symptoms after inoculation with the bacterium or fungus. Thus, the OsRDR6-mediated RNA silencing pathway seems to participate in defense against not only viruses, but also bacterial and fungal pathogens.
KeywordsOsRDR6 shl2-rol Rice Cucumber mosaic virus Rice necrosis mosaic virus Xanthomonas oryzae pv. oryzae Magnaporthe oryzae
We are grateful to D. Murphy for checking the English in the manuscript and Md. Panna Ali for the RT-qPCR analysis. We also thank Drs. M. Mori and H. Ochiai for seeds of Sensyou and Asominori, respectively. This work was supported partly by the Program for Promotion of Basic and Applied Researches in Bio-oriented Industry and Science, the Technology Research Promotion Program for Agriculture, Forestry, Fisheries and Food Industry, the Ministry of Education, Culture, Sports, Science and Technology of Japan (Grant-in-Aid for Scientific Research for Scientific Research (C), no. 24580065 to MN and (B), no. 26292026 to KK) and by grants from the Ministry of Agriculture, Forestry and Fisheries of Japan (Rice Genome Project to MN).
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