Protection induced by volatile limonene against anthracnose disease in Arabidopsis thaliana
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d-Limonene induces expression of PDF1.2 gene and resistance against Colletotrichum higginsianum in Arabidopsis ecotype Col-0. The limonene-induced expression of PDF1.2 was diminished in jar1-1 (jasmonate resistant 1-1) plants, indicating that the response of Arabidopsis depends on jasmonic acid (JA)-regulated defense signaling pathway. In fact, systemic induction of PDF1.2 was confirmed when the Arabidopsis plants harboring the PDF1.2 promotor::GUS were exposed to limonene. A similar protective effect was also observed on Japanese mustard spinach (Brassica rapa var. perviridis) challenged with C. higginsianum, suggesting that plants are capable of recognizing gaseous limonene and activate disease resistance.
KeywordsArabidopsis thaliana Colletotrichum higginsianum d-Limonene Plant defensin (PDF1.2) Systemic resistance Volatile
We gratefully acknowledge Drs. Yoshihiro Narusaka and Mari Narusaka (Research Institute of Biological Sciences Okayama, Okayama, Japan) for their gift of Arabidopsis transgenic seeds harboring the PDF1.2 promotor-GUS (β-glucuronidase) fusion gene as the reporter gene. This research was in part supported by the Research Grant for Encouragement of Students to KF from the Graduate School of Natural Science and Technology, Okayama University, Japan.
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