Protection induced by volatile limonene against anthracnose disease in Arabidopsis thaliana
- 276 Downloads
d-Limonene induces expression of PDF1.2 gene and resistance against Colletotrichum higginsianum in Arabidopsis ecotype Col-0. The limonene-induced expression of PDF1.2 was diminished in jar1-1 (jasmonate resistant 1-1) plants, indicating that the response of Arabidopsis depends on jasmonic acid (JA)-regulated defense signaling pathway. In fact, systemic induction of PDF1.2 was confirmed when the Arabidopsis plants harboring the PDF1.2 promotor::GUS were exposed to limonene. A similar protective effect was also observed on Japanese mustard spinach (Brassica rapa var. perviridis) challenged with C. higginsianum, suggesting that plants are capable of recognizing gaseous limonene and activate disease resistance.
KeywordsArabidopsis thaliana Colletotrichum higginsianum d-Limonene Plant defensin (PDF1.2) Systemic resistance Volatile
We gratefully acknowledge Drs. Yoshihiro Narusaka and Mari Narusaka (Research Institute of Biological Sciences Okayama, Okayama, Japan) for their gift of Arabidopsis transgenic seeds harboring the PDF1.2 promotor-GUS (β-glucuronidase) fusion gene as the reporter gene. This research was in part supported by the Research Grant for Encouragement of Students to KF from the Graduate School of Natural Science and Technology, Okayama University, Japan.
- Narusaka M, Minami T, Iwabuchi C, Hamasaki T, Takasaki S, Kawamura K, Narusaka Y (2015) Yeast cell wall extract induces disease resistance against bacterial and fungal pathogens in Arabidopsis thaliana and Brassica crop. PLoS One 10:e0115864. doi: 10.1371/journal.pone.0115864 PubMedCentralCrossRefPubMedGoogle Scholar
- Singh G, Singh OP (2002) Chemistry of essential oils of Citrus species. Nat Prod Rad 1:8–21Google Scholar
- Toyoda K, Ikeda S, Morikawa J, Hirose M, Maeda A, Suzuki T, Inagaki Y, Ichinose Y, Shiraishi T (2013) The Medicago truncatula–Mycosphaerella pinodes interaction: a new pathosystem for dissecting fungal-suppressor-mediated disease susceptibility in plants. J Gen Plant Pathol 79:1–11CrossRefGoogle Scholar