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Journal of General Plant Pathology

, Volume 81, Issue 3, pp 211–217 | Cite as

Involvement of ThSNF1 in the development and virulence of biocontrol agent Trichoderma harzianum

  • Luis Galarza
  • Yasunori Akagi
  • Kazumi Takao
  • Esther Peralta
  • Efrén Santos
  • Motoichiro KodamaEmail author
Fungal Diseases
  • 257 Downloads

Abstract

Trichoderma harzianum, a biocontrol agent for various plant pathogens, is known to degrade fungal cell walls; this mycoparasitism is believed to require secretion of cell-wall-degrading enzymes against host pathogens. In this study, we identified a homologue of yeast SNF1 (sucrose nonfermenting 1) encoding protein kinase in T. harzianum (ThSNF1) by draft genome sequencing of strain T36. Targeted gene disruption of ThSNF1 was performed using the PEG method with fusion PCR products. Growth of mutant ΔThSNF1 was markedly less than for the wild-type strain on minimal medium with chitin as a carbon source. The mutant exhibited reduced expression of the genes encoding chitinase and polygalacturonase and markedly reduced spore production. Mycoparasitism against plant pathogens such as Fusarium oxysporum f. sp. cubense (Panama disease) and Fusarium graminearum (Fusarium head blight) was clearly impaired in the mutant. The results suggest that ThSNF1 is critical for asexual development, utilization of certain carbon sources and virulence on fungi, and is therefore important for the biocontrol ability of T. harzianum.

Keywords

Trichoderma harzianum ThSNF1 Mycoparasitism Biocontrol Cell-wall-degrading enzymes 

Notes

Acknowledgments

We thank H. Suga for providing the fungal strains and R. P. Oliver for providing the transformation vector. This work was supported by the Global COE Program “Advanced Utilization of Fungus/Mushroom Resources for Sustainable Society in Harmony with Nature,” MEXT, Japan. We thank the National Secretary of Higher Education, Science, Technology and Education of Ecuador and the Biotechnology Research Center of Ecuador, Higher Polytechnic College of the Littoral CIBE-ESPOL for supporting this research.

Supplementary material

10327_2015_590_MOESM1_ESM.tiff (756 kb)
Supplementary material 1 (TIFF 755 kb)

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Copyright information

© The Phytopathological Society of Japan and Springer Japan 2015

Authors and Affiliations

  • Luis Galarza
    • 1
  • Yasunori Akagi
    • 2
  • Kazumi Takao
    • 1
  • Esther Peralta
    • 3
  • Efrén Santos
    • 3
  • Motoichiro Kodama
    • 1
    Email author
  1. 1.The United Graduate School of Agricultural SciencesTottori UniversityTottoriJapan
  2. 2.Faculty of AgricultureTottori UniversityTottoriJapan
  3. 3.Biotechnology Research Center of EcuadorHigher Polytechnic College of the Littoral CIBE-ESPOL. Campus Gustavo GalindoGuayaquilEcuador

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