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Journal of General Plant Pathology

, Volume 81, Issue 3, pp 201–210 | Cite as

Characterization of Trichoderma species isolated in Ecuador and their antagonistic activities against phytopathogenic fungi from Ecuador and Japan

  • Luis Galarza
  • Yasunori Akagi
  • Kazumi Takao
  • Chang Sun Kim
  • Nitaro Maekawa
  • Akihiro Itai
  • Esther Peralta
  • Efrén Santos
  • Motoichiro KodamaEmail author
Fungal Diseases

Abstract

Native Trichoderma spp. were isolated from agricultural fields in several regions of Ecuador. These isolates were characterized via morphological observation as well as molecular phylogenetic analysis based on DNA sequences of the rDNA internal transcribed spacer region, elongation factor-1α gene and RNA polymerase subunit II gene. Fifteen native Trichoderma spp. were identified as T. harzianum, T. asperellum, T. virens and T. reesei. Some of these strains showed strong antagonistic activities against several important pathogens in Ecuador, such as Fusarium oxysporum f. sp. cubense (Panama disease) and Mycosphaerella fijiensis (black Sigatoka) on banana, as well as Moniliophthora roreri (frosty pod rot) and Moniliophthora perniciosa (witches’ broom disease) on cacao. The isolates also showed inhibitory effects on in vitro colony growth tests against Japanese isolates of Fusarium oxysporum f. sp. lycopersici, Alternaria alternata and Rosellinia necatrix. The native Trichoderma strains characterized here are potential biocontrol agents against important pathogens of banana and cacao in Ecuador.

Keywords

Trichoderma spp. Biocontrol Fusarium oxysporum f. sp. cubense Mycosphaerella fijiensis Moniliophthora roreri Moniliophthora perniciosa 

Notes

Acknowledgments

We thank T. Arie, T. Yasuda and D. G. Gilchrist for providing the fungal strains. This work was supported by the Global COE Program “Advanced Utilization of Fungus/Mushroom Resources for Sustainable Society in Harmony with Nature,” MEXT, Japan. We thank the National Secretary of Higher Education, Science, Technology and Education of Ecuador and the Biotechnology Research Center of Ecuador, Higher Polytechnic College of the Littoral CIBE-ESPOL for supporting this research.

Supplementary material

10327_2015_587_MOESM1_ESM.tiff (920 kb)
Supplementary material 1 (TIFF 920 kb) Supplementary Fig. 1. Dual cultures to test antagonism of Trichoderma harzianum strains (T1, T3, T15, T19, T20 and T36) against pathogenic fungi and single cultures of each fungal pathogen after 10 days at 25 °C. A 5-mm-diameter mycelial disk of the respective fungi was placed on plates with the pathogen on lower side, Trichoderma spp. on upper side. (Foc) Fusarium oxysporum f. sp. cubense (Fo-01), (Mf) Mycosphaerella fijiensis (Ec-01), (Mr) Moniliophthora roreri (Cp-01), (Mp) Mo. perniciosa (MrEO-1), (Fol) F. oxysporum f. sp. lycopersici (Chz1-A), (Aa) Alternaria alternata tomato pathotype (As-27), (Rn) Rosellinia necatrix (ES-0601).
10327_2015_587_MOESM2_ESM.tiff (1.1 mb)
Supplementary material 2 (TIFF 1114 kb) Supplementary Fig. 2. Dual cultures to test antagonism of Trichoderma harzianum strains (T2, T4, T5, T9, T10, T13 and T18) against pathogenic fungi and single cultures of each fungal pathogen after 10 days at 25°C. A 5-mm-diameter mycelial disk of the respective fungi was placed on plates with the pathogen on lower side, Trichoderma spp. on upper side. Pathogens: (Foc) Fusarium oxysporum f. sp. cubense (Fo-01), (Mf) Mycosphaerella fijiensis (Ec-01), (Mr) Moniliophthora roreri (Cp-01), (Mp) Mo. perniciosa (MrEO-1), (Fol) F. oxysporum f. sp. lycopersici (Chz1-A), (Aa) Alternaria alternata tomato pathotype (As-27), (Rn) Rosellinia necatrix (ES-0601).
10327_2015_587_MOESM3_ESM.tiff (983 kb)
Supplementary material 3 (TIFF 983 kb) Supplementary Fig. 3. Dual cultures to test antagonism of Trichoderma reesei strain (T29) and T. virens strain (T43) against pathogenic fungi and single cultures of each fungal pathogen after 10 days at 25°C. A 5-mm-diameter mycelial disk of the respective fungi was placed on plates with the pathogen on lower side, Trichoderma spp. on upper side. Pathogens: (Foc) Fusarium oxysporum f. sp. cubense (Fo-01), (Mf) Mycosphaerella fijiensis (Ec-01), (Mr) Moniliophthora roreri (Cp-01), (Mp) Mo. perniciosa (MrEO-1), (Fol) F. oxysporum f. sp. lycopersici (Chz1-A), (Aa) Alternaria alternata tomato pathotype (As-27), (Rn) Rosellinia necatrix (ES-0601).

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Copyright information

© The Phytopathological Society of Japan and Springer Japan 2015

Authors and Affiliations

  • Luis Galarza
    • 1
  • Yasunori Akagi
    • 2
  • Kazumi Takao
    • 1
  • Chang Sun Kim
    • 3
  • Nitaro Maekawa
    • 3
  • Akihiro Itai
    • 2
  • Esther Peralta
    • 4
  • Efrén Santos
    • 4
  • Motoichiro Kodama
    • 1
    Email author
  1. 1.The United Graduate School of Agricultural SciencesTottori UniversityTottoriJapan
  2. 2.Faculty of AgricultureTottori UniversityTottoriJapan
  3. 3.Fungus/Mushroom Resource and Research Center, Faculty of AgricultureTottori UniversityTottoriJapan
  4. 4.Biotechnology Research Center of EcuadorHigher Polytechnic College of the Littoral CIBE-ESPOLGuayaquilEcuador

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