Overexpression of OsHAP2E for a CCAAT-binding factor confers resistance to Cucumber mosaic virus and Rice necrosis mosaic virus
- 378 Downloads
HAP (CBF/NF-Y) transcription factors have important functions in regulating plant growth, development, and stress responses. In this study, we examined whether the endogenous gene OsHAP2E and the GUS transgene driven by the promoter of OsHAP2E respond to virus infection. RT-PCR analyses showed OsHAP2E expression was induced after inoculation with Cucumber mosaic virus (CMV) or Rice necrosis mosaic virus (RNMV). After inoculating OsHAP2E::GUS-transgenic plants with either virus, the levels of GUS expression increased significantly. The expression levels of GUS or OsHAP2E reached a plateau 5 days after inoculation of rice with CMV, which paralleled the accumulation of CMV RNA level. Furthermore, transgenically over-expressed lines of OsHAP2E (OsHAP2E-OX) had lower levels of CMV and RNMV RNAs than in in nontransgenic control plants. The OsHAP2E-OX lines developed no significant symptoms from RNMV while control plants had yellowing and stunting. These results suggested that OsHAP2E is induced by virus infection and contributes to resistance against viral pathogens.
KeywordsCucumber mosaic virus CCAAT GUS Heme activator protein 2E (HAP2E) Rice Rice necrosis mosaic virus
We thank Drs. C. Masuta and T. Tachibana for the infested soil with P. graminis-carrying RNMV and the use of Retoratome, respectively. We are also grateful to D. Murphy for English editing of the manuscript. This work was supported by the Program for Promotion of Basic and Applied Researches in Bio-oriented Industry, and the Ministry of Education, Culture, Sports, Science and Technology of Japan [Grant-in-Aid for Scientific Research for Scientific Research (C) and (B), No. 24580065 to MN and No. 26292026 to KK] and by grants from the Ministry of Agriculture, Forestry and Fisheries of Japan (Rice Genome Project RMP-2113 to MN; Green Technology Project EF-1001 and EF-1004 to HI).
- Alam MM, Tanaka T, Nakamura H, Ichikawa H, Kobayashi K, Yaeno T, Yamaoka N, Shimomoto K, Takayama K, Nishina H, Nishiguchi M (2014b) Overespression of a rice heme activator protein gene (OsHAP2E) confers resistance to pathogens, salinity and drought, and increases photosynthesis and tiller number. Plant Biotechnol J. doi: 10.1111/pbi.12239 PubMedGoogle Scholar
- Chen H, Kobayashi K, Yamaoka N, Nishiguchi M (2011) Further analysis of rice RNA-dependent RNA polymerase 1 (OsRDR1) in RNA silencing and antiviral defense. Jpn J Phytopathol 77:59Google Scholar
- Fujii S (1978) Studies on rice necrosis mosaic virus. Special Bull Okayama Pref Agric Expt Stn 69:81Google Scholar
- Hanada K, Tochihara H (1987) Attenuated CMV. In: Umeya K, Kato H (eds) Microorganisms useful in agriculture: their use and perspectives (in Japanese). Youken-do Press, Tokyo, pp 58–70Google Scholar
- Inouye T, Fujii S (1977) Rice necrosis mosaic virus. CMI/AAB Descr Pl Viruses No. 172Google Scholar
- Lee WK, Seo HY (1992) On eighteen aphids (Tribe aphidini) occurring in Korea with a description of a new species (Homoptera: Aphididae). Korean J Ent 22:101–111Google Scholar
- Nishiguchi M, Shimono M, Eguchi Y, Okuizumi H, Yazaki J, Nakamura K, Fujii F, Shimbo K, Shimatani Z, Nagata Y, Hashimoto A, Ohta T, Sato Y, Honda S, Iwano M, Yamamoto K, Sakata K, Sasaki T, Kishimoto N, Kikuchi S (2004) Microarray analysis of gene expression in rice treated with probenazole, a resistance inducer, in special reference to blast disease. In: Kawasaki S (ed) Rice blast: interaction with rice and control. Kluwer, Dordrecht, pp 145–154CrossRefGoogle Scholar
- Zenbayashi R, Hanada K, Tochihara H (1987) Control of tomato mosaic disease by spray inoculation of attenuated strains of cucumber mosaic virus (in Japanese). Annu Rep Kanto-Tosan Plant Prot Soc 34:50–51Google Scholar
- Zhou GH, Chen JB, Chen SM (1990) Comparative identification of wheat spindle streak mosaic virus from different plants in China (in Chinese with English summary). Acta Phytopathol Sin 20:107–110Google Scholar