First report of black scurf on carrot caused by binucleate Rhizoctonia AG-U
- 335 Downloads
Black scurf on carrot roots was found in Hokkaido, Japan, in 2010. An isolate of a binucleate Rhizoctonia was obtained from sclerotia on the root surface. This isolate was identified as anastomosis group (AG)-U based on cultural characteristics, hyphal fusions and the sequence of ribosomal DNA-internal transcribed spacer region. The AG-U isolate caused black scurf symptoms on carrot roots in an inoculation test. The reference isolate of Rhizoctonia solani AG-2-2 IV, which causes carrot root rot, a disease with symptoms that differ from the black scurf symptom. This is the first report of carrot black scurf caused by binucleate Rhizoctonia AG-U.
KeywordsAG-U Binucleate Rhizoctonia Black scurf Carrot
We thank Dr. S. Kuninaga (Health Sciences University of Hokkaido, Japan) for providing the reference isolates and N. Takada (Hokkaido Prefectural Oshima Agricultural Extension Center, Japan) for sampling support.
- Carling DE (1996) Grouping in Rhizoctonia solani by hyphal anastomosis reaction. In: Sneh B, Jabaji-Hare S, Neate S, Dijst G (eds) Rhizoctonia species taxonomy, molecular biology, ecology, pathology and disease control. Kluwer, Dordrecht, pp 37–47Google Scholar
- Nagai Y, Fukatsu R (1971) Root rot symptom of carrot caused by Rhizoctonia solani (abstract in Japanese). Ann Phytopathol Soc Jpn 37:369Google Scholar
- Naito S, Mohamad D, Nasution A, Purwanti H (1993) Soil-borne diseases and ecology of pathogens on soybean roots in Indonesia. JARQ 26:247–253Google Scholar
- Sugawara K (2010) Studies on the diagnosis and identification of new fungal diseases of ornamental plants occurring in Yamagata Prefecture, and attempts to use low-chemical-input control methods against some soil-borne diseases in sandy soil. Spec Bull Agric Res Yamagata 2:38–39Google Scholar
- White TJ, Bruns T, Lee S, Taylor JW (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic Press, San Diego, pp 315–322Google Scholar