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Transmission and detection of toria [Brassica rapa L. subsp. dichotoma (Roxb.)] phyllody phytoplasma and identification of a potential vector

  • Mehdi Azadvar
  • Virendra Kumar BaranwalEmail author
  • D. K. Yadava
Bacterial and Phytoplasma Diseases

Abstract

Phyllody disease associated with 16SrIX phytoplasma was observed in the range of 4.1–11% in 10 different lines of toria [Brassica rapa L. subsp. dichotoma (Roxb.)] in experimental fields of the Indian Agricultural Research Institute, New Delhi, India during 2008 and 2009. The toria phyllody (TP) phytoplasma was detected in all the symptomatic and 13.3% of asymptomatic toria plants by nested PCR. The phytoplasma was detected in midrib, flower part, siliquae, stem, and root of infected plants as well as seeds. TP was transmitted by grafting and by dodder to toria and nine other rapeseed/mustard species as confirmed by nested PCR. However, symptoms of phytoplasma infection were induced only in toria, yellow sarson [Brassica rapa L. subsp. trilocularis (Roxb.)], brown sarson [Brassica rapa L. subsp. sarson (Prain)], rapeseed (B. napus subsp. oleifera), and rocket or taramira (Eruca sativa) but not in mustard (B. juncea), black mustard (B. nigra), Ethiopian mustard (B. carinata), B. tournefortii and white mustard (Sinapis alba). Transmission of TP phytoplasma to periwinkle (Catharanthus roseus) was successful only through dodder, but no transmission to tomato (Lycopersicon esculentum) or brinjal (Solanum melongena) was found. TP phytoplasma was detected in Laodelpax striatellus, an abundant planthopper in toria fields, which indicates that this planthopper may be a potential vector for TP phytoplasma.

Keywords

Phytoplasma Rapeseed mustard Small brown planthopper Toria Transmission 

Notes

Acknowledgments

We thank Dr. V.V. Ramamurthy, Principal Scientist Division of Entomology, IARI, New Delhi, India for planthopper identification. We are also grateful to Dr. R.K. Jain, Head, Division of Plant Pathology, IARI, New Delhi, India for providing facilities and encouragement for this work.

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Copyright information

© The Phytopathological Society of Japan and Springer 2011

Authors and Affiliations

  • Mehdi Azadvar
    • 1
    • 3
  • Virendra Kumar Baranwal
    • 1
    Email author
  • D. K. Yadava
    • 2
  1. 1.Plant Virology Unit, Division of Plant PathologyIndian Agricultural Research InstituteNew DelhiIndia
  2. 2.Division of GeneticsIndian Agricultural Research InstituteNew DelhiIndia
  3. 3.Plant Protection DepartmentAgricultural Research Center of Jiroft and KahnoujJiroftIran

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