Journal of General Plant Pathology

, Volume 77, Issue 3, pp 147–151 | Cite as

Novel vectors for retrotransposon-induced gene silencing in Magnaporthe oryzae

  • Ba Van Vu
  • Masako Takino
  • Toshiki Murata
  • Hitoshi Nakayashiki
Technique
First Online:
Received:
Accepted:

Abstract

Here we report a novel system to induce RNA silencing in Magnaporthe oryzae using the retrotransposon MAGGY. The gene-silencing vector pSilent-MG carries a MAGGY variant with a unique cloning site in the 3′ UTR region and a geneticin-resistance cassette as a selection marker. A gene-silencing assay with a GFP gene revealed that pSilent-MG vectors induced gene silencing at a practical level. In the resulting GFP-silenced transformants, GFP siRNAs accumulated and DNA methylation increased in the GFP-expressing gene, implying that the vector could induce both transcriptional and post-transcriptional gene silencing.

Keywords

Magnaportheoryzae Gene silencing Retrotransposon PTGS TGS 
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Notes

Acknowledgments

This work was supported in part by a Grant-in-Aid for Scientific Research from Japan Society for the Promotion of Science.

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Copyright information

© The Phytopathological Society of Japan and Springer 2011

Authors and Affiliations

  • Ba Van Vu
    • 1
  • Masako Takino
    • 1
  • Toshiki Murata
    • 1
  • Hitoshi Nakayashiki
    • 1
  1. 1.Laboratory of Plant Pathology, Graduate school of Agricultural ScienceKobe UniversityNadaJapan

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