First report of chlorotic and necrotic disease caused by Tobacco rattle virus on Capsicum annuum in Japan
- 181 Downloads
Severe chlorosis and necrosis were observed on Capsicum annuum in Ibaraki Prefecture, Japan. A virus strain was isolated and identified as Tobacco rattle virus (TRV) by rod-shaped viral particles observed with transmission electron microscopy and by sequence analysis with a reverse transcription-polymerase chain reaction using TRV-specific primers. The disease was reproduced by back-inoculation to green pepper seedlings and their subsequent transplant into field soil containing putative nematode vectors, Trichodorus and/or Paratrichodorus. Thus, we showed that TRV is responsible for this novel disease. This report is the first of TRV infection of C. annuum in Japan.
KeywordsCapsicum annuum Tobacco rattle virus Nematode Symptom
We thank S. Nagai and Y. Matsumura, for maintaining plants and materials. We thank T. Mizukubo and H. Sakai for helpful comments on the detection of the nematodes.
- Cobb NA (1918) Estimating the nema population of soil, with special references to the sugarbeet and root-gall nemas, Heterodera schachtii Schmidt and Heterodera radicicola (Greef) Muller, and with a description of Tylencholaimus aequalis n. sp. Agric Tech Circular No. 1. USDA, Washington DC, p 48Google Scholar
- Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA (eds) (2005) Virus taxonomy. Eighth Report of the International Committee on Taxonomy of Viruses. Elsevier Academic Press, San Diego, 1259 pGoogle Scholar
- Harrison BD, Robinson DJ (1986) Tobraviruses. In: Van Regenmortel HMV, Fraenkel-Conrat H (eds) The plant viruses, vol 2. Plenum Press, New York, pp 339–369Google Scholar
- Phytopathological Society of Japan (2000) Common names of plant diseases in Japan (in Japanese). Japan Plant Protection Association, Tokyo, 857 pGoogle Scholar