Journal of General Plant Pathology

, Volume 77, Issue 2, pp 136–138 | Cite as

First report of chlorotic and necrotic disease caused by Tobacco rattle virus on Capsicum annuum in Japan

  • Yasuhiro Tomitaka
  • Taketo Fujimoto
  • Michio Nakata
  • Ryoji Ishikawa
  • Tomio Usugi
  • Shinya Tsuda
Disease Note
  • 151 Downloads

Abstract

Severe chlorosis and necrosis were observed on Capsicum annuum in Ibaraki Prefecture, Japan. A virus strain was isolated and identified as Tobacco rattle virus (TRV) by rod-shaped viral particles observed with transmission electron microscopy and by sequence analysis with a reverse transcription-polymerase chain reaction using TRV-specific primers. The disease was reproduced by back-inoculation to green pepper seedlings and their subsequent transplant into field soil containing putative nematode vectors, Trichodorus and/or Paratrichodorus. Thus, we showed that TRV is responsible for this novel disease. This report is the first of TRV infection of C. annuum in Japan.

Keywords

Capsicum annuum Tobacco rattle virus Nematode Symptom 

Notes

Acknowledgments

We thank S. Nagai and Y. Matsumura, for maintaining plants and materials. We thank T. Mizukubo and H. Sakai for helpful comments on the detection of the nematodes.

References

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Copyright information

© The Phytopathological Society of Japan and Springer 2011

Authors and Affiliations

  • Yasuhiro Tomitaka
    • 1
  • Taketo Fujimoto
    • 2
  • Michio Nakata
    • 3
  • Ryoji Ishikawa
    • 3
  • Tomio Usugi
    • 1
  • Shinya Tsuda
    • 1
  1. 1.National Agricultural Research CenterIbarakiJapan
  2. 2.Faculty of AgricultureKyoto UniversityKyotoJapan
  3. 3.Ibaraki Agriculture InstituteIbarakiJapan

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