Molecular biological studies on mechanisms involved in pathogenicity of citrus pathogens
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Apple stem grooving virus (ASGV) (formerly citrus tatter leaf virus), the type species of the genus Capillovirus in the family Flexiviridae, is the causal agent of citrus bud-union crease syndrome and apple top-working disease. Xanthomonas axonopodis pv. citri (Xac) causes citrus canker symptoms including cell hypertrophy and abnormal division leading to eventual cell death. Here we elucidated the mechanism for viral gene expression that is responsible for ASGV virulence, the plant factors essential for symptom development in response to Xac, and a plant protein candidate involved in both ASGV and Xac pathogenicity during the host–pathogen interactions.
Mechanisms for pathogenicity regulation and gene expression involved in ASGV infection
The gene expression mechanisms of ASGV have not been well clarified because of its unique genome structure composed of two overlapping ORFs. The larger ORF1 encodes an apparently chimeric polyprotein containing at least two conserved...
KeywordsCoat Protein ORF1 Protein Pectin Methyl Esterase Citrus Canker Apple Stem Groove Virus
I would like to express my gratitude to Profs. Shinji Tsuyumu, Shigetou Namba, Masashi Ugaki, and Yuichi Takikawa for their enthusiastic support and insightful suggestions. I also express my thanks to Prof. Keiichi Tomaru, Dr. Hisatoshi Kaku, Prof. Keiko Natsuaki, Prof. Gustaaf A. de Zoeten, Dr. Jihad S. Skaf, and to many other researchers including all the laboratory members for their contributions and assistance.
- Hirata H, Yamaji Y, Komatsu K, Kagiwada S, Oshima K, Okano Y, Takahashi S, Ugaki M, Namba S (2010) Pseudo-polyprotein translated from the full-length ORF1 of capillovirus is important for pathogenicity, but a truncated ORF1 protein without variable and CP regions is sufficient for replication. Virus Res 152:1–9CrossRefPubMedGoogle Scholar