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Journal of General Plant Pathology

, Volume 76, Issue 2, pp 132–142 | Cite as

A TonB-dependent transducer is responsible for regulation of pathogenicity-related genes in Xanthomonas axonopodis pv. citri

  • Luqman Qurata Aini
  • Hisae Hirata
  • Shinji Tsuyumu
Bacterial and Phytoplasma Diseases

Abstract

The TonB-dependent transducer is required for plant pathogenicity in several plant pathogenic bacteria. In this study, we investigated the role of a putative TonB-dependent transducer, XAC4131, in Xanthomonas axonopodis pv. citri, the causal agent of citrus canker disease. A mutation in the XAC4131 gene caused a delay in the elicitation of the hypersensitive reaction in tobacco leaves. However, the pathogenicity in citrus leaves was similar to that of wild type. In hrp-inducing medium, XAC4131 controlled the expression of the hrp regulatory gene hrpG. Also, XAC4131 was involved in expression of the adjacent genes rpoE XAC4129 and XAC4130, which encode RpoE(XAC4129) and FecR-like protein, respectively. The results suggest that XAC4131 controls the expression of hrpG indirectly, probably via rpoE XAC4129 and XAC4130. Furthermore, we also demonstrated that transcription of rpoE XAC4129 , XAC4130 and XAC4131 seems to be regulated by the Fur protein.

Keywords

Citrus canker HR hrp Hypersensitive reaction TonB-dependent transducer Xanthomonas axonopodis pv. citri 

Notes

Acknowledgments

We thank Drs. U. Bonas, J. Leach, A. Yamazaki and A. Bogdanove for their encouragements and strains. This research was supported in part by a Grant-in-Aid (No.17108001) and by a grant for Promotion in Science (No.13073) from the Ministry of Education, Culture, Sports, Science and Technology of Japan.

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Copyright information

© The Phytopathological Society of Japan and Springer 2010

Authors and Affiliations

  • Luqman Qurata Aini
    • 1
    • 4
  • Hisae Hirata
    • 2
  • Shinji Tsuyumu
    • 1
    • 2
    • 3
  1. 1.Graduate School of Science and TechnologyShizuoka UniversityShizuokaJapan
  2. 2.Faculty of AgricultureShizuoka UniversityShizuokaJapan
  3. 3.Institute for Genetic Research and TechnologyShizuoka UniversityShizuokaJapan
  4. 4.Faculty of AgricultureBrawijaya UniversityMalangIndonesia

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