Journal of General Plant Pathology

, Volume 76, Issue 1, pp 37–42

Haplotype diversity and molecular evolution of the rice Pikm locus for blast resistance

  • Ikuo Ashikawa
  • Jianzhong Wu
  • Takashi Matsumoto
  • Ryuji Ishikawa
Fungal Diseases

Abstract

Pikm-specific rice blast resistance is conferred by a combination of two genes that have a nucleotide-binding site and a leucine-rich repeat (LRR), Pikm1-TS and Pikm2-TS. To study the evolution of these genes, we investigated the allele diversity of their LRR regions in 16 elite rice cultivars and 35 landraces. Both phylogenetic trees were characterized by a deep bifurcation that separated two major clades of alleles. A high level of polymorphism was detected exclusively between these clades and not within each clade. This two-clade structure commonly observed for the two genes suggests that these genes have evolved together through bidirectional differentiation.

Keywords

Rice blast Resistance gene Pikm Evolution Allele variation 

Supplementary material

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Supplementary Figure S1 (PPT 246 kb)
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Supplementary Figure S4 (PPT 144 kb)
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Supplementary Table S1 (DOC 54 kb)
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Supplementary Table S2 (DOC 53 kb)

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Copyright information

© The Phytopathological Society of Japan and Springer 2009

Authors and Affiliations

  • Ikuo Ashikawa
    • 1
  • Jianzhong Wu
    • 2
  • Takashi Matsumoto
    • 2
  • Ryuji Ishikawa
    • 3
  1. 1.NARO, National Institute of Crop ScienceTsukubaJapan
  2. 2.Plant Genome Research UnitNational Institute of Agrobiological SciencesTsukubaJapan
  3. 3.Faculty of Agriculture and Life ScienceHirosaki UniversityHirosakiJapan

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