Journal of General Plant Pathology

, Volume 75, Issue 2, pp 125–130 | Cite as

Genetic diversity and pathogenicity of Fusarium oxysporum isolated from wilted Welsh onion in Japan

  • Maha Laksha Mudiyanselage Chandrika Dissanayake
  • Rumi Kashima
  • Shuhei Tanaka
  • Shin-ichi Ito
Fungal Diseases

Abstract

Thirty isolates of Fusarium oxysporum from wilted Welsh onion plants were examined for their diversity in nucleotide sequences of the ribosomal DNA (rDNA) intergenic spacer (IGS) region and for pathogenicity with regard to five Welsh onion cultivars. Phylogenetic analysis based on the IGS sequences revealed polyphyletic origins of the isolates and a relationship between phylogeny and pathogenicity; low virulence isolates differed genetically from those with high and moderate virulence. Mating type analysis revealed that all F. oxysporum isolates were MAT1-1 idiomorphs, suggesting that the pathogens may be clonal in the fields examined.

Keywords

Allium fistulosum Fusarium wilt Mating type Phylogeny 

Notes

Acknowledgments

We thank Mr. Masataka Funahashi, Nissan Seed, Nishinomiya, Japan, for providing wilted Welsh onion samples. This work was supported in part by Nissan Seed Foundation.

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Copyright information

© The Phytopathological Society of Japan and Springer 2009

Authors and Affiliations

  • Maha Laksha Mudiyanselage Chandrika Dissanayake
    • 1
  • Rumi Kashima
    • 2
  • Shuhei Tanaka
    • 2
  • Shin-ichi Ito
    • 2
  1. 1.The United Graduate School of Agricultural SciencesTottori UniversityTottoriJapan
  2. 2.Department of Biological and Environmental Sciences, Faculty of AgricultureYamaguchi UniversityYamaguchiJapan

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