First report of a Tomato chlorotic dwarf viroid disease on tomato plants in Japan
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A viroid disease causing chlorosis of leaves and dwarfism was found on commercial tomato plants in Hiroshima Prefecture, Japan. Grafting of stems from infected plants onto healthy plants resulted in the same symptoms on the healthy plants. Small RNAs were isolated from infected plant tissue and caused identical symptoms by 3–4 week after mechanical inoculation of tomato seedlings. Nucleotide sequencing indicated that the causal pathogen was Tomato chlorotic dwarf viroid (TCDVd) sharing 98% nucleotide sequence identity with that of a Canadian isolate reported previously. This description is the first of TCDVd infection of tomato plants in Japan.
KeywordsSolanum lycopersicon Mechanical transmission PCR Tomato chlorotic dwarf viroid
The authors are grateful to S. Matsuura, Hiroshima Prefectural Agriculture Research Center, for helpful comments and discussion. This study was supported in part by a Grant-in-Aid of the Research Project for Utilizing Advanced Technologies in Agriculture, Forestry and Fisheries, administered by the Ministry of Agriculture, Forestry and Fisheries in Japan.
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