Journal of General Plant Pathology

, Volume 73, Issue 2, pp 89–95 | Cite as

Cloning of DNA fragments specific to the pathotype and race of Verticillium dahliae

  • Toshiyuki Usami
  • Shu Ishigaki
  • Hiroko Takashina
  • Yuko Matsubara
  • Yoshimiki Amemiya
FUNGAL DISEASES
First Online:
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Accepted:

Abstract

Japanese isolates of Verticillium dahliae, a causal agent of wilt disease in many plants, are classifiable into pathotypes based on their pathogenicity. Because these pathotypes are morphologically indistinguishable, establishing a rapid identification method is very important for the control of this pathogen in Japan. For cloning DNA fragments that are useful for identification and specific detection of V. dahliae pathotypes, we performed random amplified polymorphic DNA (RAPD) analyses using various isolates. One polymerase chain reaction (PCR) product, E10-U48, was specific to isolates pathogenic to sweet pepper. The other product, B68-TV, was specific to race 1 of isolates pathogenic to tomato. The specificity of these sequences was confirmed by genomic Southern hybridization. Further analyses revealed that the region peripheral to B68-TV obtained from the genomic DNA library includes the sequence specific to all isolates pathogenic to tomato (races 1 and 2). Moreover, sequence tagged site (STS) primers designed from B68-TV and its peripheral region showed race-specific and pathotype-specific amplification in a PCR assay. The probes and primers obtained in this study are likely to be useful tools for the identification and specific detection of pathotypes and races of V. dahliae.

Key words

Verticillium dahliae Pathotype Race RAPD DNA marker PCR 
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Copyright information

© The Phytopathological Society of Japan and Springer-Verlag Tokyo 2007

Authors and Affiliations

  • Toshiyuki Usami
    • 1
  • Shu Ishigaki
    • 1
  • Hiroko Takashina
    • 1
  • Yuko Matsubara
    • 1
  • Yoshimiki Amemiya
    • 1
  1. 1.Faculty of HorticultureChiba UniversityMatsudoJapan

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