Journal of General Plant Pathology

, Volume 72, Issue 5, pp 273–283 | Cite as

PCR-based differentiation of Fusarium oxysporum ff. sp. lycopersici and radicis-lycopersici and races of F. oxysporum f. sp. lycopersici

FUNGAL DISEASES

Abstract

The pathogenic type (form and race) of Fusarium oxysporum, which generates wilt symptoms on tomato, was rapidly identified with a polymerase chain reaction (PCR)-based technique. We compared the partial nucleotide sequences of endo polygalacturonase (pg1) and exo polygalacturonase (pgx4) genes from isolates of F. oxysporum ff. sp. lycopersici (FOL) and radicis-lycopersici (FORL) from Japan and designed specific primer sets (uni, sp13, sp23, and sprl) based on the nucleotide differences that appeared among the pathogenic types. PCR with the uni primer set amplified a 670∼672-bp fragment from all isolates of FOL and FORL. With the sp13 primer set, an amplicon of 445 bp was obtained only from isolates of FOL race 1 and 3. With the sp23 primer set, a 518-bp fragment was obtained from isolates of FOL race 2 and 3. The sprl primer set yielded a 947-bp fragment from isolates of FORL, but not from FOL. A combination of amplifications with these primer sets effectively differentiated the pathogenic types of F. oxysporum in tomato.

Key words

Diagnosis Forma specialis Identification Polygalacturonase Nucleotide polymorphism Tomato 

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Copyright information

© The Phytopathological Society of Japan and Springer-Verlag Tokyo 2006

Authors and Affiliations

  1. 1.Tokyo University of Agriculture and Technology (TUAT)TokyoJapan
  2. 2.Saitama Prefectural Agriculture and Forestry Research CenterSaitamaJapan

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