Journal of General Plant Pathology

, Volume 72, Issue 1, pp 34–39 | Cite as

Flagella-mediated motility is required for biofilm formation by Erwinia carotovora subsp. carotovora

BACTERIAL AND PHYTOPLASMA DISEASES

Abstract

To elucidate the role of flagella in biofilm formation by Erwinia carotovora subsp. carotovora EC1, we used a nonflagellate, nonmotile mutant (ΔfliC) and a flagellate, nonmotile mutant (ΔmotA). A biofilm-inducing medium, which contains the yeast peptone (YP) medium plus the salts of M-63 minimal medium, supported biofilm formation to a greater extent than either the YP or Luria Bertani (LB) medium alone. We demonstrated that both the ΔfliC and ΔmotA mutants greatly reduced their ability to form a biofilm on the surface of the wells of polyvinyl chloride (PVC) microtiter plates. The inability of both mutants to form biofilm on the PVC surface was further confirmed with phase-contrast microscopy. Both aflagellate (ΔfliC) and flagellate (ΔmotA) nonmotile mutants were equally defective in attachment to the PVC surface. The treatment of bacteria with the protonophore carbonyl cyanide m-chlorophenylhydrazone (CCCP), which inhibits the motility of this organism, reduced greatly the biofilm formation. Based on these results, flagella-mediated motility may play an important role in biofilm formation of E. carotovora subsp. carotovora EC1.

Key words

Erwinia carotovora subsp. carotovora EC1 Biofilm formation Motility Carbonyl cyanide m-chlorophenylhydrazone ΔfliC ΔmotA 

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Copyright information

© The Phytopathological Society of Japan and Springer-Verlag Tokyo 2006

Authors and Affiliations

  1. 1.Faculty of Agriculture, Shizuoka UniversityGifu United Graduate School of AgricultureShizuokaJapan
  2. 2.Laboratory of Plant Pathology, Faculty of AgricultureShizuoka UniversityShizuokaJapan

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