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Journal of General Plant Pathology

, Volume 72, Issue 1, pp 6–15 | Cite as

Reactive oxygen species (ROS) generation and ROS-induced lipid peroxidation are associated with plasma membrane modifications in host cells in response to AK-toxin I from Alternaria alternata Japanese pear pathotype

  • Naoto Shimizu
  • Naoki Hosogi
  • Gang-Su Hyon
  • Shan Jiang
  • Kanako Inoue
  • Pyoyun ParkEmail author
FUNGAL DISEASES

Abstract

AK-toxin I caused plasma membrane modifications with plasma membrane-derived membrane fragments only in sensitive Japanese pear tissues. H2O2 generation was abundant in both the membrane fragments and the plasma membranes of the toxin-treated sensitive tissues. Whether lipid peroxidation was induced in plasma membranes of the toxin-treated sensitive tissues was examined biochemically and histochemically. Lipid peroxidation was caused only in the toxin-treated sensitive tissues or the toxin-treated plasma membrane-enriched fractions from sensitive young pear fruits. The results indicated that the peroxidation was probably induced by reactive oxygen species in the modified plasma membranes by action of toxin, suggesting that peroxidation is closely associated with plasma membrane modifications.

Key words

AK-toxin I Lipid peroxidation H2O2 generation Ultrastructure Japanese pear plants Alternaria alternata Japanese pear pathotype 

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Copyright information

© The Phytopathological Society of Japan and Springer-Verlag Tokyo 2006

Authors and Affiliations

  • Naoto Shimizu
    • 1
  • Naoki Hosogi
    • 1
  • Gang-Su Hyon
    • 1
  • Shan Jiang
    • 1
  • Kanako Inoue
    • 1
  • Pyoyun Park
    • 1
    Email author
  1. 1.Laboratory of Stress Cytology, The Graduate School of Science and TechnologyKobe UniversityKobeJapan

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