Journal of General Plant Pathology

, Volume 72, Issue 1, pp 20–25

Molecular analysis and virus transmission tests place Olpidium virulentus, a vector of Mirafiori lettuce big-vein virus and tobacco stunt virus, as a distinct species rather than a strain of Olpidium brassicae

FUNGAL DISEASES

Abstract

The rDNA-ITS sequences of ten single-sporangium isolates of Olpidium virulentus (a noncrucifer strain of Olpidium brassicae), which transmits Mirafiori lettuce big-vein virus (MLBVV) and tobacco stunt virus (TStV), were compared with those of six single-sporangium isolates of O. brassicae. The sequence similarity within isolates of O. virulentus or O. brassicae was almost identical (98.5%–100.0%), but was low between the two species (79.7%–81.8%). In a phylogenetic analysis of the rDNA-ITS region, O. virulentus and O. brassicae fell into two distinct clusters, indicating that O. virulentus, a vector of MLBVV and TStV, is a distinct species rather than a strain of O. brassicae.

Key words

Olpidium virulentus Olpidium brassicae rDNA-ITS Taxonomy 

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Copyright information

© The Phytopathological Society of Japan and Springer-Verlag Tokyo 2006

Authors and Affiliations

  1. 1.National Agricultural Research Center for Western RegionZentsujiJapan
  2. 2.Kunisada Plant Breeding StationKaneko SeedsGunmaJapan
  3. 3.Policy Planning and Evaluation DivisionMinister's Secretariat, MAFFTokyoJapan

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