Environmental Chemistry Letters

, Volume 17, Issue 3, pp 1413–1420 | Cite as

Microbial fuel cell-induced production of fungal laccase to degrade the anthraquinone dye Remazol Brilliant Blue R

  • Marta Filipa Simões
  • Alfredo Eduardo Maiorano
  • Jonas Gomes dos Santos
  • Luciana Peixoto
  • Rodrigo Fernando Brambilla de Souza
  • Almir Oliveira Neto
  • António Guerreiro Brito
  • Cristiane Angélica OttoniEmail author
Original Paper


The anthraquinone dye Remazol Brilliant Blue R is largely used in the textile industry. However, its removal from wastewaters is costly and complex. Many methods have been tested to solve this ecological problem, but there is still a need for efficient methods. We propose here an alternative use of a two-chambered microbial fuel cell (MFC), fuelled with domestic wastewater in the anodic chamber, to degrade a simulated textile dye effluent made of Remazol Brilliant Blue R inoculated with an immobilised fungal strain, Pleurotus ostreatus URM 4809, as a laccase producer, in the cathodic chamber. The MFC showed continuous synthesis of laccase in the cathodic chamber, which, in turn, promoted the rapid decolourisation, of more than 86% of the textile dye effluent. The yield was further increased by the addition of glycerol. Electrochemical monitoring also indicated an increase in power density and current density. After 20 days of MFC operation, 62.1% of organic matter was removed in the anodic compartment, thus leaving the effluent with a much lower toxicity.


Microbial fuel cell Pleurotus ostreatus Laccase Remazol Brilliant Blue R Vigna radiata 



Authors would like to acknowledge the technician and financial support of Programa Novos Talentos provided by the Instituto de Pesquisa Tecnológica do Estado de São Paulo (IPT) and Instituto de Estudos Avançados do Mar (IEAMar).

Compliance with ethical standards

Conflicts of interest

There are no conflicts to declare.

Supplementary material

10311_2019_876_MOESM1_ESM.docx (25 kb)
Supplementary material 1 (DOCX 25 kb)
10311_2019_876_MOESM2_ESM.docx (49 kb)
Supplementary material 2 (DOCX 49 kb)


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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Marta Filipa Simões
    • 1
  • Alfredo Eduardo Maiorano
    • 2
  • Jonas Gomes dos Santos
    • 2
  • Luciana Peixoto
    • 3
  • Rodrigo Fernando Brambilla de Souza
    • 4
  • Almir Oliveira Neto
    • 4
  • António Guerreiro Brito
    • 5
  • Cristiane Angélica Ottoni
    • 2
    • 6
    • 7
    • 8
    Email author
  1. 1.Biology DepartmentEdge Hill UniversityLancashireUK
  2. 2.Laboratório de Biotecnologia IndustrialInstituto de Pesquisas Tecnológicas do Estado de São PauloSão PauloBrazil
  3. 3.Centre of Biological EngineeringUniversity of MinhoBragaPortugal
  4. 4.Centro de Célula a Combustível e HidrogênioInstituto de Pesquisas Energéticas e NuclearesSão PauloBrazil
  5. 5.Department of Biosystems Sciences and Engineering, Institute of AgronomyUniversity of LisbonLisbonPortugal
  6. 6.São Paulo State University (UNESP)São VicenteBrazil
  7. 7.Instituto de Estudos Avançados do Mar (IEAMar/UNESP)São VicenteBrazil
  8. 8.Bioscience InstituteSão Paulo State UniversitySão VicenteBrazil

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