Nitrate reductase for nitrate analysis in water
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Nitrate analysis in water is one of the most frequently applied methods in environmental chemistry. Current methods for nitrate are generally based on toxic substances. Here, we show that a viable alternative method is to use the enzyme nitrate reductase. The key to applying this Green Chemistry solution for nitrate analysis is plentiful, inexpensive, analytical grade enzyme. We demonstrate that recombinant Arabidopsis nitrate reductase, expressed in the methylotrophic yeast Pichia pastoris, is a highly effective catalyst for nitrate analysis at 37°C. Recombinant production of enzyme ensures consistent quality and provides means to meet the needs of environmental chemistry.
KeywordsNitrate reductase Recombinant expression Nitrate Green chemistry Water Enzymes
We thank Prof. N. M. Crawford for supplying the Arabidopsis NaR2 containing plasmid. We also thank Troy Kinnunen-Skidmore and Michael J. B. Campbell for assistance with the experiments. This work was supported, in part, by the Michigan Tech Research Fund.