Gynäkologische Endokrinologie

, Volume 1, Issue 1, pp 35–41 | Cite as

Zehn Jahre ICSI

Teil 1: Indikationen, Grenzen, Hintergründe männlicher Subfertilität
Übersicht
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Zusammenfassung

Die intrazytoplasmatische Spermieninjektion (ICSI) hat die Behandlung der männlichen Subfertilität revolutioniert. Der vorliegende Artikel soll 10 Jahre nach Einführung in die klinische Routine die Möglichkeiten der ICSI das Indikationsspektrum und die Erfolge darstellen. Ebenso sollen aber auch kritisch die Grenzen der Technik sowie Überlegungen zur Sicherheit und nicht zuletzt Daten zur Gesundheit der nach ICSI geborenen Kinder beleuchtet werden.

Schlüsselwörter

Intrazytoplasmatische Spermieninjektion Männliche Subfertilität Indikation 

Abstract

Intracytoplasmic sperm injection (ICSI) has revolutionized the treatment of male subfertility. This article presents the possibilities, spectrum of indications, and achievements of ICSI, which was introduced into routine clinical practice 10 years ago. A critical review serves to elucidate the technical limitations, considerations on safety, and data on the health of children born after ICSI.

Keywords

Intracytoplasmic sperm injection Male subfertility Indication 
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Literatur

  1. 1.
    Aboulghar MA, Mansour RT, Serour GI et al. (1996) Prospective controlled randomized study of in vitro fertilization versus intracytoplasmic sperm injection in the treatment of tubal factor infertility with normal semen parameters. Fertil Steril 66:753–756Google Scholar
  2. 2.
    Benadiva CA, Nulsen J, Siano L et al. (1999) Intracytoplasmic sperm injection overcomes previous fertilization failure with conventional in vitro fertilization. Fertil Steril 72:1041–1044Google Scholar
  3. 3.
    Bhattacharya S, Hamilton MP, Shaaban M, Khalaf Y, Seddler M, Ghobara T, Braude P, Kennedy R, Rutherford A, Hartshorne G, Templeton A (2001) Conventional in-vitro fertilisation versus intracytoplasmic sperm injection for the treatment of non-male-factor infertility: a randomised controlled trial. Lancet 357:2075–2079Google Scholar
  4. 4.
    Bonduelle M, Aytoz A, Wilikens A et al. (1998) Prospective follow-up study of 1987 children born after intracytoplasmic sperm injection (ICSI). In: Filicori M, Flamigni C (eds) Treatment of infertility: the new frontiers. Communications Media for Education, Inc., New Jersey, pp 445–461Google Scholar
  5. 5.
    Bonduelle M, Liebaers I, Deketelaere V et al. (2002) Neonatal data on a cohort of 2889 infants born after ICSI (1991–1999) and of 2995 infants born after IVF (1983–1999). Hum Reprod 17:671–694Google Scholar
  6. 6.
    Bourgain C, Nagy ZP, De Zutter H et al. (1998) Ultrastructure of gametes after intracytoplasmic sperm injection. Hum Reprod 13 [Suppl 1]:107–116Google Scholar
  7. 7.
    Chillon M, Casals T, Mercier B et al. (1995) Mutations in the cystic fibrosis gene in patients with congenital absence of the vas deferens. N Engl J Med 332:1475–1480Google Scholar
  8. 8.
    Danan C, Sternberg D, Van Steirteghem A et al. (1999) Evaluation of parental mitochondrial inheritance in neonates born after intracytoplasmic sperm injection. Am J Hum Genet 65:463–473Google Scholar
  9. 9.
    De Braekeleer M, Dao TN (1991) Cytogenetic studies in male infertility: a review. Hum Reprod 6:245–250Google Scholar
  10. 10.
    Devroey P, Nagy P, Tournaye H et al. (1996) Outcome of intracytoplasmic sperm injection with testicular spermatozoa in obstructive and non-obstructive azoospermia. Hum Reprod 11:1015–1018Google Scholar
  11. 11.
    Doyle P, Beral V, Maconochie N (1992) Preterm delivery, low birthweight and small-for-gestational-age in liveborn singleton babies resulting from in-vitro fertilization. Hum Reprod 7:425–428Google Scholar
  12. 12.
    Dumoulin JCM, Coonen E, Bras M et al. (2000) Comparison of in-vitro development of embryos originating from either conventional in-vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI). Hum Reprod 15:402–409Google Scholar
  13. 13.
    Engel W, Murphy D, Schmid M (1996) Are there genetic risks associated with microassisted reproduction? Hum Reprod 11:2359–2370Google Scholar
  14. 14.
    Ferguson-Smith MA, Lennox B, Mack WS, Stewart JSS (1957) Klinefelter's syndrome: frequency and testicular morphology in relation to nuclear sex. Lancet 2:167Google Scholar
  15. 15.
    Friedler S, Raziel A, Strassburger D et al. (2001) Outcome of ICSI using fresh and cryopreserved-thawed testicular spermatozoa in patients with non-mosaic Klinefelter's syndrome. Hum Reprod 16:2616–2620Google Scholar
  16. 16.
    Gabrielsen A, Petersen K, Mikkelsen AL, Lindenberg S (1996) Intracytoplasmic sperm injection does not overcome an oocyte defect in previous fertilization failure with conventional in-vitro fertilization and normal spermatozoa. Hum Reprod 11:1963–1965Google Scholar
  17. 17.
    Griffiths TA, Murdoch AP, Herbert M (2000) Embryonic development in vitro is compromised by the ICSI procedure. Hum Reprod 15:1592–1596Google Scholar
  18. 18.
    Hansen M, Kurinczuk JJ, Bower C, Webb S (2002) The risk of major birth defects after intracytoplasmic sperm injection and in vitro fertilization. N Engl J Med 346:725–730Google Scholar
  19. 19.
    Hardarson T, Lundin K, Hamberger L (2000) The position of the metaphase II spindle cannot be predicted by the location of the first polar body in the human oocyte. Hum Reprod 15:1372–1376Google Scholar
  20. 20.
    Hewitson L, Dominko T, Takahashi D et al. (1999) Unique checkpoints during the first cell cycle of fertilization after intracytoplasmic sperm injection in rhesus monkeys. Nature Med 5:431–433Google Scholar
  21. 21.
    Hewitson L, Martinovich C, Simerly C et al. (2000) Is round spermatid injection (ROSI) a therapy for male infertility? ROSI in the rhesus monkey is unsuccessful. Fertil Steril [Suppl 1] 74:S68Google Scholar
  22. 22.
    Hewitson L, Martinovich C, Takahashi D, Schatten G (2000) Elongated spermatid injection (ELSI) in the rhesus monkey. Fertil Steril [Suppl 1] 74:S67Google Scholar
  23. 23.
    Hewitson L, Simerly CR, Tengowski MW et al. (1996) Microtubule and chromatin configurations during Rhesus intracytoplasmic sperm injection: successes and failures. Biol Reprod 55:271–280Google Scholar
  24. 24.
    Hewitson L, Takahashi D, Dominko T et al. (1998) Fertilization and embryo development to blastocysts after intracytoplasmic sperm injection in the rhesus monkey. Hum Reprod 13, 3449–3455.Google Scholar
  25. 25.
    Houshmand,M., Holme,E., Hanson,C., Wennerholm,U.B. & Hamberger,L. 1997. Is paternal mitochondrial DNA transferred to the offspring following intracytoplasmic sperm injection? J Assist Reprod Genet 14:223–227Google Scholar
  26. 26.
    Iritani A (1991) Micromanipulation of gametes for in vitro assisted fertilization. Mol Reprod Dev 28:199–207Google Scholar
  27. 27.
    Jun JH, Lim CK, Kim JW et al. (1999) Comparison of fertilization and embryonic development between conventional insemination and ICSI treatment in the sibling oocytes of non-male factor infertility. Fertil Steril [Suppl 1] 72:S6Google Scholar
  28. 28.
    Kastrop PM, Weima SM, Van Kooij RJ, Te Velde ER (1999) Comparison between intracytoplasmic sperm injection and in-vitro fertilization (IVF) with high insemination concentration after total fertilization failure in a previous IVF attempt. Hum Reprod 14:65–69Google Scholar
  29. 29.
    Kent-First MG, Kol S, Muallem A et al. (1996) The incidence and possible relevance of Y-linked microdeletions in babies born after intracytoplasmic sperm injection and their infertile fathers. Mol Hum Reprod 2:943–950Google Scholar
  30. 30.
    Khamsi F, Yavas Y, Roberge S et al. (2001) Intracytoplasmic sperm injection increased fertilization and good-quality embryo formation in patients with non-male factor indications for in vitro fertilization: a prospective randomized study. Fertil Steril 75:342–347Google Scholar
  31. 31.
    Kimura Y, Yanagimachi R (1995) Intracytoplasmic sperm injection in the mouse. Biol Reprod 52:709–720Google Scholar
  32. 32.
    Kimura Y, Yanagimachi R (1995) Mouse oocytes injected with testicular spermatozoa or round spermatids can develop into normal offspring. Development 121:2397–2405Google Scholar
  33. 33.
    Küpker W, Schlegel PN, Al-Hasani S et al. (2000) Use of frozen thawed testicular sperm for intracytoplasmic sperm injection. Fertil Steril 73:453–458Google Scholar
  34. 34.
    Lamb VK, Scott-Brown M, Marchington DR et al. (1999) Does intracytoplasmic sperm injection (ICSI) result in the inheritance of paternal mitochondrial DNA? Hum Reprod (Abstract book) 14:124Google Scholar
  35. 35.
    Lanzendorf SE, Maloney MK, Veeck LL et al. (1988) A preclinical evaluation of pronuclear formation by microinjection of human spermatozoa into human oocytes. Fertil Steril 49:835–842Google Scholar
  36. 36.
    Liu J, Nagy Z, Joris H et al. (1995) Successful fertilization and establishment of pregnancies after intracytoplasmic sperm injection in patients with globozoospermia. Hum Reprod 10:626–629Google Scholar
  37. 37.
    Ludwig M, Schröder AK, Diedrich K (2001) Impact of intracytoplasmic sperm injection on the activation and fertilization process of oocytes. RBMonline 3:228–238Google Scholar
  38. 38.
    Ludwig M, Strik D, Al-Hasani S, Diedrich K (1999) No transfer in a planned ICSI cycle: we cannot overcome some basic rules of human reproduction. Europ J Obstet Gynecol Reprod Biol 87:3–11Google Scholar
  39. 39.
    Luetjens CM, Payne C, Schatten G (1999) Non random chromosome positioning in human sperm and sex chromosome anomalies following intracytoplasmic sperm injection. Lancet 353:1240Google Scholar
  40. 40.
    Menezo Y, Barak V (2000) Comparison between day-2 embryos obtained either from ICSI or resulting from short term insemination IVF: influence of maternal age. Hum Reprod 15:1776–1780Google Scholar
  41. 41.
    Meschede D, Lemcke B, Behre HM et al. (2000) Clustering of male infertility in the families of couples treated with intracytoplasmic sperm injection. Hum Reprod 15:1604–1608Google Scholar
  42. 42.
    Miller KF, Falcone T, Goldberg JM, AttaranM (1998) Previous fertilization failure with conventional in vitro fertilization is associated with poor outcome of intracytoplasmic sperm injection. Fertil Steril 69:242–245Google Scholar
  43. 43.
    Moreno C, Mercader A, Ruiz A et al. (1998) Preimplantation development outcome of embryos after in-vitro fertilization is superior to that after intracytoplasmic sperm injection. Hum Reprod (Abstract book) 13:164Google Scholar
  44. 44.
    Nagy ZP, Liu J, Joris H et al. (1995) The result of intracytoplasmic sperm injection is not related to any of the three basic sperm parameters. Hum Reprod 10:1123–1129Google Scholar
  45. 45.
    Nagy ZP, Verheyen G, Liu J et al. (1995) Results of 55 intracytoplasmic sperm injection cycles in the treatment of male-immunological infertility. Hum Reprod 10:1775–1780Google Scholar
  46. 46.
    Ng SC, Bongso A, Ratnam SS (1991) Microinjection of human oocytes: a technique for severe oligoasthenoteratozoospermia. Fertil Steril 56:1117–1123Google Scholar
  47. 47.
    Ng SC, Sathananthan AH, Bongso TA et al. (1990) Subzonal transfer of multiple sperm (MIST) into early human embryos. Mol Reprod Dev 26:253–260Google Scholar
  48. 48.
    Palermo G, Joris H, Devroey P, Van Steirteghem AC (1992) Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte. Lancet 340:17–18Google Scholar
  49. 49.
    Reijo R, Lee TY, Salo P et al. (1995) Diverse spermatogenic defects in humans caused by Y chromosome deletions encompassing a novel RNA-binding protein gene. Nature Genetics 10:383–393Google Scholar
  50. 50.
    Ruiz A, Remohi J, Minguez Y et al. (1997) The role of in vitro fertilization and intracytoplasmic sperm injection in couples with unexplained infertility after failed intrauterine insemination. Fertil Steril 68:171–173Google Scholar
  51. 51.
    Sbracia M, Torroni A, Sellitto D et al. (2000) Paternal mitochondrial DNA in preimplantation embryos. Hum Reprod (Abstract book) 15:81Google Scholar
  52. 52.
    Sbracia S, Cozza G, Grasso JA et al. (1996) Semen parameters and sperm morphology in men in unexplained recurrent spontaneous abortion, before and during a 3 year follow-up period. Hum Reprod 11:117–120Google Scholar
  53. 53.
    Schieve LA, Meikle SF, Ferre C et al. (2002) Low and very low birth weight in infants conceived with use of assisted reproductive technology. N Engl J Med 346:731–737Google Scholar
  54. 54.
    Schröder AK, Diedrich K, Ludwig M (2001) Fertilization and preimplantation development after intracytoplasmic sperm injection. Reprod Biomed Online 3 3:247Google Scholar
  55. 55.
    Schulze W, Thoms F, Knuth UA (1999) Testicular sperm extraction: comprehensive analysis with simultaneously performed histology in 1418 biopsies from 766 subfertile men. Hum Reprod 14 [Suppl 1]:82–96Google Scholar
  56. 56.
    Shoukir Y, Chardonnens D, Campana A, Sakkas D (1998) Blastocyst development from supernumerary embryos after intracytoplasmic sperm injection: a paternal influence? Hum Reprod 13:1632–1637Google Scholar
  57. 57.
    Silber SJ, Nagy ZP, Liu J et al. (1994) Conventional in-vitro fertilisation versus intracytoplasmic sperm injection for patients requiring microsurgical sperm aspiration. Hum Reprod 9:1705–1709Google Scholar
  58. 58.
    Silva CP, Kommineni K, Oldenbourg R, Keefe DL (1999) The first polar body does not predict accurately the location of the metaphase II meiotic spindle in mammalian oocytes. Fertil Steril 71:719–721Google Scholar
  59. 59.
    St John J, Sakkas D, Dimitriadi K et al. (2000) Failure of elimination of paternal mitochondrial DNA in abnormal embryos. Lancet 355:200Google Scholar
  60. 60.
    Staessen C, Camus M, Clasen K et al. (1999) Conventional in-vitro fertilization versus intracytoplasmic sperm injection in sibling oocytes from couples with tubal infertility and normozoospermic semen. Hum Reprod 14:2474–2479Google Scholar
  61. 61.
    Steptoe PC, Edwards RG (1978) Birth after the reimplantation of a human embryo. Lancet 2:366Google Scholar
  62. 62.
    Strömberg B, Dahlquist G, Ericson A et al. (2002) Neurological sequelae in children born after in-vitro fertilisation: a population based study. Lancet 359:461–465Google Scholar
  63. 63.
    Sutovsky P, Hewitson L, Simerly CR et al. (1996) Intracytoplasmic sperm injection for Rhesus monkey fertilization results in unusual chromatin, cytoskeletal, and membrane events, but eventually leads to pronuclear development and sperm aster assembly. Hum Reprod 11:1703–1712Google Scholar
  64. 64.
    Terada Y, Luetjens CM, Sutovsky P, Schatten G (2000) Atypical decondensation of the sperm nucleus, delayed replication of the male genome, and sex chromosome positioning following intracytoplasmic human sperm injection (ICSI) into the golden hamster egg. Does ICSI itself introduce chromosome anomalies? Fertil Steril 74:454–460Google Scholar
  65. 65.
    Tesarik J, Sousa M (1994) Comparison of Ca response in human oocytes fertilized by subzonal insemination and by intracytoplasmatic sperm injection. Fertil Steril 62:1197–1204Google Scholar
  66. 66.
    Tesarik J, Sousa M, Testart J (1994) Human oocyte activation after intracytoplasmatic sperm injection. Hum Reprod 9:511–518Google Scholar
  67. 67.
    Tiepolo L, Zuffardi O (1976) Localization of factors controlling spermatogenesis in the nonfluorescent portion of the human Y chromosome long arm. Hum Genet 34:119–124Google Scholar
  68. 68.
    Tournaye H, Devroey P, Camus M et al. (1992) Comparison of in-vitro fertilization in male and tubal infertility: a 3 year survey. Hum Reprod 7:218–222Google Scholar
  69. 69.
    Trokoudes KM, Danos N, Kalogirou L et al. (1995) Pregnancy with spermatozoa from a globozoospermic man after intracytoplasmic sperm injection treatment. Hum Reprod 10:880–882Google Scholar
  70. 70.
    Urman B, Alatas C, Aksoy S et al. (2002) Transfer at the blastocyst stage of embryos derived from testicular round spermatid injection. Hum Reprod 17:741–743Google Scholar
  71. 71.
    Van Steirteghem A, Nagy P, Joris H et al. (1996) The development of intracytoplasmic sperm injection. Hum Reprod 1:59–72Google Scholar
  72. 72.
    Van Steirteghem AC, Liu J, Joris H et al. (1993) Higher success rate by intracytoplasmic sperm injection than by subzonal insemination. Report of a second series of 300 consecutive treatment cycles. Hum Reprod 8:1055–1060Google Scholar
  73. 73.
    Van Steirteghem AC, Nagy Z, Joris H et al. (1993) High fertilization and implantation rates after intracytoplasmic sperm injection. Hum Reprod 8:1061–1066Google Scholar
  74. 74.
    Vicdan K, Isik AZ (1999) Intracytoplasmic sperm injection is not associated with poor outcome in couples with normal semen parameters and previous idiopathic fertilization failure in conventional in vitro fertilization. Eur J Obstet Gynecol Reprod Biol 87:87–90Google Scholar
  75. 75.
    Wang WH, Hackett RJ, Meng L, Keefe DL (1999) Spindle observation and its relationship with fertilization after ICSI in living human oocytes. Fertil Steril [Suppl 1] 72:S1Google Scholar
  76. 76.
    Yuzpe AA, Liu Z, Fluker MR (2000) Rescue intracytoplasmic sperm injection (ICSI)-salvaging in vitro fertilization (IVF) cycles after total or near-total fertilization failure. Fertil Steril 73:1115–1119Google Scholar

Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  1. 1.Bereich Reproduktionsmedizin und gynäkologische EndokrinologieKlinik für Frauenheilkunde und Geburtshilfe, Universitätsklinikum Lübeck
  2. 2.Reproduktionsmedizin und gynäkologische EndokrinologieEndokrinologikum HamburgHamburg

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