Journal of Industrial Microbiology & Biotechnology

, Volume 46, Issue 11, pp 1557–1568 | Cite as

Increasing l-threonine production in Escherichia coli by overexpressing the gene cluster phaCAB

  • Jianli Wang
  • Wenjian Ma
  • Yu Fang
  • Jun Yang
  • Jie Zhan
  • Shangwei Chen
  • Xiaoyuan WangEmail author
Bioenergy/Biofuels/Biochemicals - Original Paper


l-Threonine is an important branched-chain amino acid and could be applied in feed, drugs, and food. In this study, l-threonine production in an l-threonine-producing Escherichia coli strain TWF001 was significantly increased by overexpressing the gene cluster phaCAB from Ralstonia eutropha. TWF001/pFW01-phaCAB could produce 96.4-g/L l-threonine in 3-L fermenter and 133.5-g/L l-threonine in 10-L fermenter, respectively. In addition, TWF001/pFW01-phaCAB produced 216% more acetyl-CoA, 43% more malate, and much less acetate than the vector control TWF001/pFW01, and meanwhile, TWF001/pFW01-phaCAB produced poly-3-hydroxybutyrate, while TWF001/pFW01 did not. Transcription analysis showed that the key genes in the l-threonine biosynthetic pathway were up-regulated, the genes relevant to the acetate formation were down-regulated, and the gene acs encoding the enzyme which converts acetate to acetyl-CoA was up-regulated. The results suggested that overexpression of the gene cluster phaCAB in E. coli benefits the enhancement of l-threonine production.


l-Threonine production Escherichia coli Poly-3-hydroxybutyrate phaCAB Acetate 



This study was supported by the National Key R&D Program of China (2018YFA0900302), the National First-Class Discipline Program of Light Industry Technology and Engineering (LITE2018-10), the post-graduate research and practice innovation program of Jiangsu Province (KYLX15_1141), and the Collaborative Innovation Center of Jiangsu Modern Industrial Fermentation.


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Copyright information

© Society for Industrial Microbiology and Biotechnology 2019

Authors and Affiliations

  • Jianli Wang
    • 1
    • 2
  • Wenjian Ma
    • 1
    • 2
  • Yu Fang
    • 1
    • 2
  • Jun Yang
    • 1
    • 3
  • Jie Zhan
    • 1
    • 2
  • Shangwei Chen
    • 1
  • Xiaoyuan Wang
    • 1
    • 2
    • 3
    Email author
  1. 1.State Key Laboratory of Food Science and TechnologyJiangnan UniversityWuxiChina
  2. 2.International Joint Laboratory on Food SafetyJiangnan UniversityWuxiChina
  3. 3.Key Laboratory of Industrial Biotechnology, Ministry of Education, School of BiotechnologyJiangnan UniversityWuxiChina

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