Lipidomics characterization of the alterations of Trichoderma brevicompactum membrane glycerophospholipids during the fermentation phase

  • Yunfan Bai
  • Yuran Gao
  • Xin Lu
  • Huiyu WangEmail author
Metabolic Engineering and Synthetic Biology - Original Paper


The biological membrane lipid composition has been demonstrated to greatly influence the secretion of secondary metabolites. This study was conducted to investigate the periodical alterations of whole cellular lipids and their associations with secondary products in Trichoderma brevicompactum. An electrospray ionization–mass spectrometry-based lipidomics strategy was used to acquire the metabolic profiles of membrane lipids during fermentation. Univariate analyses showed that most fungi glycerophospholipids were significantly altered at the early phase compared with the late phase. In addition, correlation analyses showed high correlations between phosphatidylcholine alterations and fermentation duration. In addition, the fermentation-associated alterations of phosphatidylcholines were found to be in accordance with the degrees of unsaturation of acyl-chains. Harzianum A reached a maximum on the 12th day, while trichodermin and 6-pentyl-2H-pyran-2-one showed the highest abundances on the 9th day, both of which were inclined to correlate with the alterations of phosphatidylcholines and phosphatidylethanolamines, respectively. These findings demonstrated that the alterations of the membrane lipid species in Trichoderma spp. were associated with the fermentation phases and might influence the secretion of specific secondary products, which may be useful in studying the optimization of secondary products in Trichoderma spp.


Trichoderma brevicompactum Metabolic profile Lipidomics Secondary product Glycerophospholipid 



This study was funded by the Science and Technology Project of Qiqihar City (SFGG-201543) and the Doctoral Scientific Fund Project (QY2015B-03). We sincerely express our appreciation to the Forest Department in Northeast Forest University for donating the Trichoderma brevicompactum strain to this study.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

10295_2019_2152_MOESM1_ESM.tif (8.3 mb)
Fig. S1 Score plot of PCA model constructed with 128 lipid species in T. brevicompactum mycelia. Black, red, green, blue, yellow boxes indicate T. brevicompactum mycelia collected on the 3rd day, the 6th day, the 9th day, the 12th day, and the 15th day, respectively
10295_2019_2152_MOESM2_ESM.tif (8 mb)
Fig. S2 Corresponding validation plot of PLS-DA model. Green dots are R2 values, while blue dots are Q2 values
10295_2019_2152_MOESM3_ESM.tif (5.6 mb)
Fig. S3 DVCs constructed with PEs and PSs. (a) DVC of PEs; (b) DVCs of PSs. Colors and sizes of dots mean different degrees of fold-change
10295_2019_2152_MOESM4_ESM.tif (7.9 mb)
Fig. S4 Spearman correlation analyses between PCs, PEs, PSs and (a) 6-Pentyl-2H-pyran-2-one; (b) harzianum A; (c) trichodermin. Green, yellow, and purple dots indicate PC, PE, PS lipid species, respectively. Red dots indicate the means of correlation coefficients
10295_2019_2152_MOESM5_ESM.tif (3.7 mb)
Fig. S5 Spearman correlation analyses between growth phases and (a) different lipid categories (PC, PE, PS). The numbers of lipid species with correlation coefficients > 0.8 represented. Purple, red, pink, blue, green represent LPCs, LPEs, PCs, PEs, and PSs, respectively; (b) three secondary metabolites (trichoderma, harzianum A, 6-pentyl pyrone). The correlation coefficients were shown in the table
10295_2019_2152_MOESM6_ESM.doc (296 kb)
Table S1 Basic information of 128 lipid species in T. brevicompactum mycelia


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Copyright information

© Society for Industrial Microbiology and Biotechnology 2019

Authors and Affiliations

  1. 1.School of Life Science and TechnologyHarbin Institute of TechnologyHarbin CityChina
  2. 2.School of PharmacyQiqihar Medical UniversityQiqihar CityChina

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