Biological conversion of propane to 2-propanol using group I and II methanotrophs as biocatalysts
- 43 Downloads
Propane is the main component of liquefied petroleum gas and is derived from crude oil processing. Methanotrophic bacteria can convert various alkanes using methane monooxygenase enzyme to primary alcohols. These are further oxidized to various aldehydes by alcohol dehydrogenases or methanol dehydrogenases. In this study, 2-propanol was produced from propane using the whole cells of Methylosinus trichosporium OB3b, Methylomicrobium alcaliphilum 20Z, and Methylomonas sp. DH-1 as the biocatalysts. The biocatalytic process of converting propane to 2-propanol was optimized by the use of several inhibitors and additives, such as EDTA, sodium phosphate, and sodium formate to prevent oxidation of 2-propanol to acetone and to enhance conversion of propane to propanol. The maximum titer of 2-propanol was 0.424 g/L, 0.311 g/L, and 0.610 g/L for Methylomonas sp. DH-1, M. alcaliphilum 20Z, and M. trichosporium OB3b whole cells, respectively. These results showed that type I and type II methanotrophs could be used as the potent biocatalyst for conversion of propane to propanol.
KeywordsMethylomonas sp. DH-1 M. trichosporium OB3b Methane monooxygenase 2-Propanol Propane
This research was supported by the C1 Gas Refinery Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (2015M3D3A1A01064882).
TTN performed the experiments and prepared a draft of the manuscript. JGN and IYH reviewed and edited the manuscript. EYL coordinated the study and finalized the manuscript. All authors have read and approved the manuscript.
- 14.Hanson RS, Hanson TE (1996) Methanotrophic bacteria. Microbiol Rev 60:439–471Google Scholar
- 16.Hiu SF, Zhu C, Yan R, Chen J (1987) Butanol-ethanol dehydrogenase and butanol-ethanol-isopropanol dehydrogenase: different alcohol dehydrogenases in two strains of Clostridium beijerinckii (Clostridium butylicum). Appl Environ Microbiol 53:697–703Google Scholar
- 22.Kalyuzhnaya MG, Khmelenina V, Eshinimaev B, Sorokin D, Fuse H, Lidstrom M, Trotsenko Y (2008) Classification of halo (alkali) philic and halo (alkali) tolerant methanotrophs provisionally assigned to the genera Methylomicrobium and Methylobacter and emended description of the genus Methylomicrobium. Int J Syst Evol Microbiol 58:591–596CrossRefGoogle Scholar
- 35.Nguyen HH, Shiemke AK, Jacobs SJ, Hales BJ, Lidstrom ME, Chan SI (1994) The nature of the copper ions in the membranes containing the particulate methane monooxygenase from Methylococcus capsulatus (Bath). J Biol Chem 269:14995–15005Google Scholar
- 36.Patel RN, Hou CT, Laskin AI, Felix A (1982) Microbial oxidation of hydrocarbons: properties of a soluble methane monooxygenase from a facultative methane-utilizing organism, Methylobacterium sp. strain CRL-26. Appl Environ Microbiol 44:1130–1137Google Scholar
- 41.Semrau JD, Jagadevan S, DiSpirito AA, Khalifa A, Scanlan J, Bergman BH, Freemeier BC, Baral BS, Bandow NL, Vorobev A (2013) Methanobactin and MmoD work in concert to act as the ‘copper-switch’ in methanotrophs. Environ Microbiol 15:3077–3086Google Scholar