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Corn steep liquor as a nutritional source for biocementation and its impact on concrete structural properties

  • Sumit Joshi
  • Shweta Goyal
  • M. Sudhakara Reddy
Environmental Microbiology - Original Paper
  • 474 Downloads

Abstract

Microbial-induced carbonate precipitation (MICP) has a potential to improve the durability properties and remediate cracks in concrete. In the present study, the main emphasis is placed upon replacing the expensive laboratory nutrient broth (NB) with corn steep liquor (CSL), an industrial by-product, as an alternate nutrient medium during biocementation. The influence of organic nutrients (carbon and nitrogen content) of CSL and NB on the chemical and structural properties of concrete structures is studied. It has been observed that cement-setting properties were unaffected by CSL organic content, while NB medium influenced it. Carbon and nitrogen content in concrete structures was significantly lower in CSL-treated specimens than in NB-treated specimens. Decreased permeability and increased compressive strength were reported when NB is replaced with CSL in bacteria-treated specimens. The present study results suggest that CSL can be used as a replacement growth medium for MICP technology at commercial scale.

Keywords

Biomineralization Curing Organic admixture Concrete Corn steep liquor Carbon and nitrogen content 

Notes

Acknowledgements

The authors are thankful to Science and Engineering Research Board (SERB), Department of Science & Technology, Government of India, India, for the financial support under the research Project No. SB/S3/CEE/0063/2013.

Supplementary material

10295_2018_2050_MOESM1_ESM.pdf (211 kb)
Supplementary material 1 (PDF 210 kb)

References

  1. 1.
    Achal V, Mukherjee A, Basu PC, Reddy MS (2009) Lactose mother liquor as an alternative nutrient source for microbial concrete production by Sporosarcina pasteurii. J Ind Microbiol Biotechnol 36:433–438CrossRefPubMedGoogle Scholar
  2. 2.
    Achal V, Mukherjee A, Goyal S, Reddy MS (2012) Corrosion prevention of reinforced concrete with microbial calcite precipitation. ACI Mater J 109:157–164Google Scholar
  3. 3.
    Achal V, Mukherjee A, Reddy MS (2010) Biocalcification by Sporosarcina pasteurii using corn steep liquor as the nutrient source. Ind Biotechnol 6:170–174CrossRefGoogle Scholar
  4. 4.
    Achal V, Mukherjee A, Reddy MS (2011) Effect of calcifying bacteria on permeation properties of concrete structures. J Ind Microbiol Biotechnol 38:1229–1234CrossRefPubMedGoogle Scholar
  5. 5.
    Achal V, Mukherjee A, Reddy MS (2011) Microbial concrete: way to enhance the durability of building structures. J Mater Civil Eng 23:730–734CrossRefGoogle Scholar
  6. 6.
    Amiri A, Bundur ZB (2018) Use of corn-steep liquor as an alternative carbon source for biomineralization in cement-based materials and its impact on performance. Constr Build Mater 165:655–662CrossRefGoogle Scholar
  7. 7.
    Aprianti SE (2016) A huge number of artificial waste material can be supplementary cementitious material (SCM) for concrete production—a review part II. J Cleaner Prod 142:4178–4194CrossRefGoogle Scholar
  8. 8.
    ASTM C1202–97 (1997) Standard test method for electrical indication of concrete's ability to resist chloride ion penetration. ASTM International, West Conshohocken, PA. http://www.astm.org/
  9. 9.
    ASTM C1585-04 (2004) Standard test method for measurement of rate of absorption of water by hydraulic-cement concretes. ASTM International, West Conshohocken, PA. http://www.astm.org/
  10. 10.
    Basheer L, Kropp J, Cleland DJ (2001) Assessment of the durability of concrete from its permeation properties: a review. Constr Build Mater 15:93–103CrossRefGoogle Scholar
  11. 11.
    Basheer PAM, Chidiact SE, Long AE (1996) Predictive models for deterioration of concrete structures. Constr Build Mater 10:27–37CrossRefGoogle Scholar
  12. 12.
    Behnood A, Tittelboom KV, De Belie N (2016) Methods for measuring pH in concrete: a review. Constr Build Mater 105:176–188CrossRefGoogle Scholar
  13. 13.
    IS: 516-1959 (1959) Indian standard methods of tests for strength of concrete. Bureau of Indian Standards, New Delhi-110002Google Scholar
  14. 14.
    IS: 8112-2013 (2013) Indian standard specification for 43 grade ordinary portland cement. Bureau of Indian Standards, New Delhi-110002Google Scholar
  15. 15.
    IS: 383-1970 (1970) Indian standard specification for coarse and fine aggregates from natural sources for concrete. Bureau of Indian Standards, New Delhi-110002Google Scholar
  16. 16.
    IS: 4031 (Part 4)-1988 (1988) Indian standard methods of physical tests for hydraulic cement. Part 4: Determination of consistency of standard cement paste. Bureau of Indian Standards, New Delhi-110002Google Scholar
  17. 17.
    IS: 4031 (Part 5)-1988 (1988) Indian standard methods of physical tests for hydraulic cement. Part 5: Determination of initial and final setting times. Bureau of Indian Standards, New Delhi-110002Google Scholar
  18. 18.
    IS: 5194-1969 (1969) Indian standard method for determination of nitrogen-Kjeldahl method. Bureau of Indian Standards, New Delhi-110002Google Scholar
  19. 19.
    Bolobova AV, Kondrashchenko VI (2000) Use of yeast fermentation waste as a biomodifier of concrete (review). Appl Biochem Microbiol 36:205–214CrossRefGoogle Scholar
  20. 20.
    Bundur ZB, Kirisits MJ, Ferron RD (2015) Biomineralized cement-based materials: impact of inoculating vegetative bacterial cells on hydration and strength. Cem Concr Res 67:237–245CrossRefGoogle Scholar
  21. 21.
    De Muynck W, Cox K, De Belie N, Verstraete W (2008) Bacterial carbonate precipitation as an alternative surface treatment for concrete. Constr Build Mater 22:875–885CrossRefGoogle Scholar
  22. 22.
    De Muynck W, De Belie N, Verstraete W (2010) Microbial carbonate precipitation in construction materials: a review. Ecol Eng 36:118–136CrossRefGoogle Scholar
  23. 23.
    Dhami NK, Reddy MS, Mukherjee A (2013) Bacillus megaterium mediated mineralization of calcium carbonate as biogenic surface treatment of green building materials. World J Microbiol Biotechnol 29:2397–2406CrossRefPubMedGoogle Scholar
  24. 24.
    Dias WPS (1995) Sorptivity testing for assessing concrete quality, in: Proc. Int. Conf. on Concrete under Severe Exposure Conditions (CONSEC’95), Spon, London, pp 433–442Google Scholar
  25. 25.
    DIN 1048 (1978) Test methods of concrete impermeability to water: part 2. Deutscher Institute Fur Normung, GermanyGoogle Scholar
  26. 26.
    Erşan YC, Da Silva FB, Boon N, Verstraete W, De Belie N (2015) Screening of bacteria and concrete compatible protection materials. Constr Build Mater 88:196–203CrossRefGoogle Scholar
  27. 27.
    Eryürük K, Suzuki D, Mizuno SY, Akatsuka T, Tsuchiya T, Yang S, Kitano H, Katayama A (2016) Decrease in hydraulic conductivity of a paddy field using biocalcification in situ. Geomicrobiol J 33:690–698CrossRefGoogle Scholar
  28. 28.
    Franzoni E, Pigino B, Pistolesi C (2013) Ethyl silicate for surface protection of concrete: performance in comparison with other inorganic surface treatments. Cem Concr Compos 44:69–76CrossRefGoogle Scholar
  29. 29.
    Huet B, L’Hostis V, Miserque F, Idrissi H (2005) Electrochemical behavior of mild steel in concrete: influence of pH and carbonate content of concrete pore solution. Electrochim Acta 51:172–180CrossRefGoogle Scholar
  30. 30.
    Joshi S, Goyal S, Mukherjee A, Reddy MS (2017) Microbial healing of cracks in concrete: a review. J Ind Microbiol Biotechnol 44:1511–1525CrossRefPubMedGoogle Scholar
  31. 31.
    Joshi S, Goyal S, Reddy MS (2018) Influence of nutrient components of media on structural properties of concrete during biocementation. Constr Build Mater 158:601–613CrossRefGoogle Scholar
  32. 32.
    Kim HK, Park SJ, Han JI, Lee HK (2013) Microbially mediated calcium carbonate precipitation on normal and lightweight concrete. Constr Build Mater 38:1073–1082CrossRefGoogle Scholar
  33. 33.
    Kristiansen B (2001) Process economics, basic biotechnology, 2nd edn. Cambridge University Press, Cambridge, pp 239–252Google Scholar
  34. 34.
    Loewus FA (1952) Improvement in anthrone method for determination of carbohydrates. Anal Chem 24:219–219CrossRefGoogle Scholar
  35. 35.
    McCarter WJ, Ezirim H, Emerson M (1992) Absorption of water and chloride into concrete. Mag Concr Res 44:31–37CrossRefGoogle Scholar
  36. 36.
    Meyer C (2009) The greening of the concrete industry. Cem Concr Compos 31:601–605CrossRefGoogle Scholar
  37. 37.
    Montaño-Salazar SM, Lizarazo-Marriaga J, Brandão PFB (2018) Isolation and potential biocementation of calcite precipitation inducing bacteria from colombian buildings. Curr Microbiol 75:256–265CrossRefPubMedGoogle Scholar
  38. 38.
    Pan X, Shi Z, Shi C, Ling TC, Li N (2017) A review on surface treatment for concrete—part 2: performance. Constr Build Mater 133:81–90CrossRefGoogle Scholar
  39. 39.
    Rodriguez-Navarro C, Jroundi F, Schiro M, Ruiz-Agudo E, González –Muñoz MT (2012) Influence of substrate mineralogy on bacterial mineralization of calcium carbonate: implications for stone conservation. Appl Environ Microbiol 78:4017–4029CrossRefPubMedPubMedCentralGoogle Scholar
  40. 40.
    Sharma N, Prasad GS, Choudhury AR (2013) Utilization of corn steep liquor for biosynthesis of pullulan, an important exopolysaccharide. Carbohydr Polym 93:95–101CrossRefPubMedGoogle Scholar
  41. 41.
    Thomas NL, Birchall JD (1983) The retarding action of sugars on cement hydration. Cem Concr Res 13:830–842CrossRefGoogle Scholar
  42. 42.
    Tremblay H, Duchesne J, Locat J, Leroueil S (2002) Influence of the nature of organic compounds on fine soil stabilization with cement. Can Geotech J 39:535–546CrossRefGoogle Scholar
  43. 43.
    Walkley A, Black IA (1934) An examination of the Degtjareff method for determining soil organic matter and a proposed modification of the chromic acid titration method. Soil Sci 37:29–38CrossRefGoogle Scholar
  44. 44.
    Williams SL, Kirisits MJ, Ferron RD (2016) Optimization of growth medium for Sporosarcina pasteurii in bio based cement pastes to mitigate delay in hydration kinetics. J Ind Microbiol Biotechnol 43:567–575CrossRefPubMedGoogle Scholar
  45. 45.
    Yeo D, Gabbai RD (2011) Sustainable design of reinforced concrete structures through embodied energy optimization. Energy Build 43:2028–2033CrossRefGoogle Scholar
  46. 46.
    Young JF (1972) A review of the mechanisms of set-retardation in Portland cement pastes containing organic admixtures. Cem Concr Res 2:415–433CrossRefGoogle Scholar

Copyright information

© Society for Industrial Microbiology and Biotechnology 2018

Authors and Affiliations

  • Sumit Joshi
    • 1
  • Shweta Goyal
    • 2
  • M. Sudhakara Reddy
    • 1
  1. 1.Department of BiotechnologyThapar Institute of Engineering & TechnologyPatialaIndia
  2. 2.Department of Civil EngineeringThapar Institute of Engineering & TechnologyPatialaIndia

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