Journal of Industrial Microbiology & Biotechnology

, Volume 43, Issue 12, pp 1641–1646 | Cite as

A whole cell biocatalyst for double oxidation of cyclooctane

  • C. A. Müller
  • A. M. Weingartner
  • A. Dennig
  • A. J. Ruff
  • H. Gröger
  • Ulrich Schwaneberg
Biocatalysis - Short Communication


A novel whole cell cascade for double oxidation of cyclooctane to cyclooctanone was developed. The one-pot oxidation cascade requires only a minimum of reaction components: resting E. coli cells in aqueous buffered medium (=catalyst), the target substrate and oxygen as environmental friendly oxidant. Conversion of cyclooctane was catalysed with high efficiency (50% yield) and excellent selectivity (>94%) to cyclooctanone. The reported oxidation cascade represents a novel whole cell system for double oxidation of non-activated alkanes including an integrated cofactor regeneration. Notably, two alcohol dehydrogenases from Lactobacillus brevis and from Rhodococcus erythropolis with opposite cofactor selectivities and one monooxygenase P450 BM3 were produced in a coexpression system in one single host. The system represents the most efficient route with a TTN of up to 24363 being a promising process in terms of sustainability as well.


Alkanes Directed evolution Oxidation Oxidoreductases Cascade reaction Monooxygenase 

Supplementary material

10295_2016_1844_MOESM1_ESM.docx (467 kb)
Supplementary material 1 (DOCX 467 kb)


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Copyright information

© Society for Industrial Microbiology and Biotechnology 2016

Authors and Affiliations

  • C. A. Müller
    • 1
  • A. M. Weingartner
    • 1
  • A. Dennig
    • 1
  • A. J. Ruff
    • 1
  • H. Gröger
    • 3
  • Ulrich Schwaneberg
    • 1
    • 2
  1. 1.Institute of BiotechnologyRWTH Aachen UniversityAachenGermany
  2. 2.DWI–Leibniz Institut für Interaktive MaterialienAachenGermany
  3. 3.Faculty of ChemistryBielefeld UniversityBielefeldGermany

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