Eighteen new oleaginous yeast species

  • Luis A. Garay
  • Irnayuli R. Sitepu
  • Tomas Cajka
  • Idelia Chandra
  • Sandy Shi
  • Ting Lin
  • J. Bruce German
  • Oliver Fiehn
  • Kyria L. Boundy-Mills


Of 1600 known species of yeasts, about 70 are known to be oleaginous, defined as being able to accumulate over 20 % intracellular lipids. These yeasts have value for fundamental and applied research. A survey of yeasts from the Phaff Yeast Culture Collection, University of California Davis was performed to identify additional oleaginous species within the Basidiomycota phylum. Fifty-nine strains belonging to 34 species were grown in lipid inducing media, and total cell mass, lipid yield and triacylglycerol profiles were determined. Thirty-two species accumulated at least 20 % lipid and 25 species accumulated over 40 % lipid by dry weight. Eighteen of these species were not previously reported to be oleaginous. Triacylglycerol profiles were suitable for biodiesel production. These results greatly expand the number of known oleaginous yeast species, and reveal the wealth of natural diversity of triacylglycerol profiles within wild-type oleaginous Basidiomycetes.


Oleaginous yeast Triacylglycerol Basidiomycete Intracellular lipid Biodiesel 



The authors are grateful to Erin Cathcart, Jennifer Lincoln, Lauren Enriquez for technical assistance. This research was funded by Grant Number U01TW008160 from the NIH Fogarty International Center, the NIH Office of Dietary Supplements, the National Science Foundation and the Department of Energy. This project was supported by the USDA Agricultural Food Research Initiative of the National Food and Agriculture, USDA, Grant Number 35621-04750. The content is solely the responsibility of the authors and does not necessarily represent the official views of the Fogarty International Center or the National Institutes of Health, the Office of Dietary Supplements, the National Science Foundation, the Department of Energy, or the Department of Agriculture. This work was supported by the Science Translation and Innovation Research (STAIR) Grant Program of the University of California Davis, and by the Consejo Nacional de Ciencia y Tecnología (CONACYT) Grant Number 291795. Funding by NIH HL113452 and NIH DK097154 (to OF) is greatly appreciated. NIH instrument funding by NIH S10-RR031630 (to OF) is acknowledged. Strains UCDFST 10-421, 10-451, 12-776, 10-1058, 10-1109, 10-453, 11-470 and 10-441 were obtained through a collaboration between UC Davis and the Government of the Republic of Indonesia.Thanks to Sarah Faulina and Sira Silaban for isolating strain Rhodotorula mucilaginosa UCDFST 13-478. All authors have agreed to submit this manuscript to the “Journal of Industrial Microbiology and Biotechnology”.

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Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

10295_2016_1765_MOESM1_ESM.pdf (91 kb)
Supplementary material 1 (PDF 90 kb)
10295_2016_1765_MOESM2_ESM.pdf (205 kb)
Supplementary material 2 (PDF 204 kb)


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Copyright information

© Society for Industrial Microbiology and Biotechnology 2016

Authors and Affiliations

  • Luis A. Garay
    • 1
  • Irnayuli R. Sitepu
    • 1
    • 2
  • Tomas Cajka
    • 3
  • Idelia Chandra
    • 1
  • Sandy Shi
    • 1
  • Ting Lin
    • 1
  • J. Bruce German
    • 4
  • Oliver Fiehn
    • 3
    • 5
  • Kyria L. Boundy-Mills
    • 1
  1. 1.Phaff Yeast Culture Collection, Department of Food Science and TechnologyUniversity of CaliforniaDavisUSA
  2. 2.Bioentrepreneurship DepartmentIndonesia International Institute for Life SciencesEast JakartaIndonesia
  3. 3.Metabolomics, UC Davis Genome CenterUniversity of California DavisDavisUSA
  4. 4.Department of Food Science and TechnologyUniversity of CaliforniaDavisUSA
  5. 5.Biochemistry Department, Faculty of ScienceKing Abdulaziz UniversityJeddahSaudi Arabia

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