Eighteen new oleaginous yeast species
- 677 Downloads
Of 1600 known species of yeasts, about 70 are known to be oleaginous, defined as being able to accumulate over 20 % intracellular lipids. These yeasts have value for fundamental and applied research. A survey of yeasts from the Phaff Yeast Culture Collection, University of California Davis was performed to identify additional oleaginous species within the Basidiomycota phylum. Fifty-nine strains belonging to 34 species were grown in lipid inducing media, and total cell mass, lipid yield and triacylglycerol profiles were determined. Thirty-two species accumulated at least 20 % lipid and 25 species accumulated over 40 % lipid by dry weight. Eighteen of these species were not previously reported to be oleaginous. Triacylglycerol profiles were suitable for biodiesel production. These results greatly expand the number of known oleaginous yeast species, and reveal the wealth of natural diversity of triacylglycerol profiles within wild-type oleaginous Basidiomycetes.
KeywordsOleaginous yeast Triacylglycerol Basidiomycete Intracellular lipid Biodiesel
The authors are grateful to Erin Cathcart, Jennifer Lincoln, Lauren Enriquez for technical assistance. This research was funded by Grant Number U01TW008160 from the NIH Fogarty International Center, the NIH Office of Dietary Supplements, the National Science Foundation and the Department of Energy. This project was supported by the USDA Agricultural Food Research Initiative of the National Food and Agriculture, USDA, Grant Number 35621-04750. The content is solely the responsibility of the authors and does not necessarily represent the official views of the Fogarty International Center or the National Institutes of Health, the Office of Dietary Supplements, the National Science Foundation, the Department of Energy, or the Department of Agriculture. This work was supported by the Science Translation and Innovation Research (STAIR) Grant Program of the University of California Davis, and by the Consejo Nacional de Ciencia y Tecnología (CONACYT) Grant Number 291795. Funding by NIH HL113452 and NIH DK097154 (to OF) is greatly appreciated. NIH instrument funding by NIH S10-RR031630 (to OF) is acknowledged. Strains UCDFST 10-421, 10-451, 12-776, 10-1058, 10-1109, 10-453, 11-470 and 10-441 were obtained through a collaboration between UC Davis and the Government of the Republic of Indonesia.Thanks to Sarah Faulina and Sira Silaban for isolating strain Rhodotorula mucilaginosa UCDFST 13-478. All authors have agreed to submit this manuscript to the “Journal of Industrial Microbiology and Biotechnology”.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- 9.Boundy-Mills K (2008) The phaff yeast culture collection has found its niche. Soc Ind Microbiol News 58:49–56Google Scholar
- 14.Cohen Z, Ratledge C (2005) Single Cell Oils. AOCS Press, ChampaignGoogle Scholar
- 15.Davis RW, Fishman D, Frank E, Wigmosta M (2012) Renewable diesel from algal lipids: an integrated baseline for cost, emissions, and resource potential from a harmonized model. Technical report ANL.ESD/12-4, NREL/TP-5100-55431, PNNL-21437. US Department of Energy Biomass ProgramGoogle Scholar
- 20.Hanna M, Isom L, Campbell J (2005) Biodiesel: current perspectives and future. J Sci Ind Res 64:854Google Scholar
- 31.Kurtzman C, Fell J, Boekhout T (2011) The yeasts: a taxonomic study, 5th edn. Elsevier, AmsterdamGoogle Scholar
- 35.Liu Y, Koh CMJ, Sun L, Hlaing MM, Du M, Peng N, Ji L (2013) Characterization of glyceraldehyde-3-phosphate dehydrogenase gene RtGPD1 and development of genetic transformation method by dominant selection in oleaginous yeast Rhodosporidium toruloides. Appl Microbiol Biotechnol 97:719–729CrossRefPubMedGoogle Scholar
- 38.McCluskey K, Bates S, Boundy-Mills K, Broggiato A, Cova A, Desmeth P, DebRoy C, Fravel D, Garrity G, del Mar Jiménez Gasco M, Joseph L, Lindner D, Lomas M, Morton J, Nobles D, Turner J, Ward T, Wertz J, Wiest A, Geiser D (2014) Meeting report: 2nd workshop of the united states culture collection network. May 19–21, 2014, State College, PA, USA. Stand Genom Sci 9:27–31Google Scholar
- 40.Moreton RS (1988) Physiology of lipid accumulating yeasts. In: Moreton RS (ed) Single Cell Oil. Longman, Harlow, UK, pp 1–32Google Scholar
- 45.Pfaller R, Leonhartsberger S (2004) Process for producing Sporidiobolus ruineniae strains with improved coenzyme Q10 production. Google PatentsGoogle Scholar
- 51.Ratledge C, Wilkinson SG (1988) Microbial lipids, vol 1. Academic Press, LondonGoogle Scholar
- 54.Schweizer M (2004) Lipids and membranes. The metabolism and molecular physiology of Saccharomyces cerevisiae. CRC Press, London, pp 140–223Google Scholar
- 56.Sitepu IR, Sestric R, Ignatia L, Levin D, Bruce German J, Gillies LA, Almada LA, Boundy-Mills KL (2013) Manipulation of culture conditions alters lipid content and fatty acid profiles of a wide variety of known and new oleaginous yeasts species. Bioresour Technol 144:360–369CrossRefPubMedGoogle Scholar
- 62.Sugiyama J, Fukagawa M, Chiu S-W, Komagata K (1985) Cellular carbohydrate composition, DNA base composition, ubiquinone systems, and Diazonium Blue B color test in the genera Rhodosporidium, Leucosporidium, Rhodotorula and related basidiomycetous yeasts. J Gen Appl Microbiol 31:519–550CrossRefGoogle Scholar
- 65.Valduga E, Ribeiro AHR, Cence K, Colet R, Tiggemann L, Zeni J, Toniazzo G (2014) Carotenoids production from a newly isolated Sporidiobolus pararoseus strain using agroindustrial substrates. Biocatal Agric Biotechnol 3:207–213Google Scholar
- 67.Woodbine M (1959) Microbial fat: micro-organisms as potential fat producers. In: Hockenhull (ed) Progress in Industrial Microbiology, vol 1. Elsevier, London, UK, pp 181–245Google Scholar