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Journal of Industrial Microbiology & Biotechnology

, Volume 42, Issue 10, pp 1319–1324 | Cite as

Efficient aspartic acid production by a psychrophile-based simple biocatalyst

  • Takahisa Tajima
  • Mai Hamada
  • Yutaka Nakashimada
  • Junichi Kato
Biocatalysis

Abstract

We previously constructed a Psychrophile-based Simple bioCatalyst (PSCat) reaction system, in which psychrophilic metabolic enzymes are inactivated by heat treatment, and used it here to study the conversion of aspartic acid from fumaric acid mediated by the activity of aspartate ammonia-lyase (aspartase). In Escherichia coli, the biosynthesis of aspartic acid competes with that of L-malic acid produced from fumaric acid by fumarase. In this study, E. coli aspartase was expressed in psychrophilic Shewanella livingstonensis Ac10 heat treated at 50 °C for 15 min. The resultant PSCat could convert fumaric acid to aspartic acid without the formation of L-malic acid because of heat inactivation of psychrophilic fumarase activity. Furthermore, alginate-immobilized PSCat produced high yields of aspartic acid and could be re-used nine times. The results of our study suggest that PSCat can be applied in biotechnological production as a new approach to increase the yield of target compounds.

Keywords

Alginate Biomass Aspartic acid Psychrophilic bacteria Immobilization 

Notes

Acknowledgments

We thank Professor Tatsuo Kurihara in Kyoto University for providing S. livingstonensis Ac10-Rifr. This study was partially supported by the Core Research for Evolutional Science and Technology (CREST) Program through the Japan Science and Technology Agency.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Society for Industrial Microbiology and Biotechnology 2015

Authors and Affiliations

  • Takahisa Tajima
    • 1
    • 2
  • Mai Hamada
    • 1
  • Yutaka Nakashimada
    • 1
    • 2
  • Junichi Kato
    • 1
  1. 1.Department of Molecular Biotechnology, Graduate School of Advanced Sciences of MatterHiroshima UniversityHigashi-HiroshimaJapan
  2. 2.Core Research of Evolutional Science and Technology (CREST)Japan Science and Technology Agency (JST)TokyoJapan

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