Advertisement

Journal of Industrial Microbiology & Biotechnology

, Volume 42, Issue 10, pp 1363–1368 | Cite as

Model-based evaluation of ferrous iron oxidation by acidophilic bacteria in chemostat and biofilm airlift reactors

  • Sirous Ebrahimi
  • Neda Faraghi
  • Maryam Hosseini
Biotechnology Methods

Abstract

This article presents a model-based evaluation of ferrous iron oxidation in chemostat and biofilm airlift reactors inoculated with a mixed culture of Acidithiobacillus ferrooxidans and Leptospirillum ferrooxidans bacteria. The competition between the two types of bacteria in the chemostat and in the biofilm airlift reactors together with the distribution of both bacteria along the biofilm thickness at different time sections has been studied. The bacterial distribution profiles along the biofilm in the airlift reactor at different time scales show that in the beginning A. ferrooxidans bacteria are dominant, but when the reactor operates for a long time the desirable L. ferrooxidans species outcompete A. ferrooxidans as a result of the low Fe2+ and high Fe3+ concentrations. The results obtained from the simulation were compared with the experimental data of continuously operated internal loop airlift biofilm reactor. The model results are in good agreement with the experimental results.

Keywords

Leptospirillum ferrooxidans Acidithiobacillus ferrooxidans AQUASIM software Ferrous iron Biofilm airlift reactor Chemostat reactor 

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Human and Animal Rights

This article does not contain any studies with human participants or animals performed by any of the authors.

References

  1. 1.
    Asai S, Konishi Y, Yabu T (1989) Kinetics of absorption of hydrogen sulfide into aqueous ferric sulfate solutions. AIChE 35(8):1271–1281CrossRefGoogle Scholar
  2. 2.
    Boon M, Brasser HJ, Hansford GS, Heijnen JJ (1999) Comparison of the oxidation kinetics of different pyrites in the presence of Thiobacillus ferrooxidans or Leptospirillum ferrooxidans. Hydrometallurgy 53(1):57–72CrossRefGoogle Scholar
  3. 3.
    Boon M, Meeder TA, Thöne C, Ras C, Heijnen JJ (1999) The ferrous iron oxidation kinetics of Thiobacillus ferrooxidans in continuous cultures. Appl Microbiol Biotechnol 51(6):820–826CrossRefGoogle Scholar
  4. 4.
    Boon M, Ras C, Heijnen JJ (1999) The ferrous iron oxidation kinetics of Thiobacillus ferrooxidans in batch cultures. Appl Microbiol Biotechnol 51(6):813–819CrossRefGoogle Scholar
  5. 5.
    Ebrahimi S, Kleerebezem R, van Loosdrecht MCM, Heijnen JJ (2003) Kinetics of the reactive absorption of hydrogen sulfide into aqueous ferric sulfate solutions. Chem Eng Sci 58(2):417–427CrossRefGoogle Scholar
  6. 6.
    Ebrahimi S, Morales FJF, Kleerebezem R, Heijnen JJ, Loosdrecht MCMv (2005) High rate acidophilic ferrous iron oxidation in a biofilm airlift reactor and the role of the carrier material. Biotechnol Bioeng 90(4):462–472CrossRefPubMedGoogle Scholar
  7. 7.
    Grishin SI, Tuovinen OH (1988) Fast kinetics of Fe2+ oxidation in packed bed reactors. Appl Environ Microbiol 54(2):3092–3100PubMedCentralPubMedGoogle Scholar
  8. 8.
    Halfmeier H, Schafer-Treffeldt W, Reuss M (1993) Potential of Thiobacillus Ferrooxidans for waste gas purification. Part 1. Kinetics of continuous ferrous iron oxidation. Appl Microbiol Biotechnol 40:416–420Google Scholar
  9. 9.
    Hansford GS (1997) Recent developments in modeling the kinetics of bioleaching. In: Rawlings DE (ed) Biomining: theory, microbes and industrial processes. Springer-Verlag and Landes Bioscience, Berlin, pp 153–175Google Scholar
  10. 10.
    Mesa MM, Macias M, Cantero D (2002) Mathematical model of the oxidation of ferrous iron by a biofilm of Thiobacillus ferrooxidans. Biotechnol Prog 18:679–685CrossRefPubMedGoogle Scholar
  11. 11.
    Pirt SJ (1982) Maintenance energy: a general model for energy-limited and energy-sufficient growth. Arch Microbiol 133(4):300–302CrossRefPubMedGoogle Scholar
  12. 12.
    Rawlings DE, Tributsch H, Hansford GH (1999) Reasons why ‘Leptospirillum’-like species rather than Thiobacillus ferrooxidans are the dominant iron-oxidizing bacteria in many commercial processes for the biooxidation of pyrite and related ores. Microbiology 145:5–13CrossRefPubMedGoogle Scholar
  13. 13.
    Roels JA (1983) Energetic and kinetics in biotechnology. Elsevier Biomedical Press, Amsterdam, pp 99–128  Google Scholar
  14. 14.
    Satoh H, Yoshizawa J, Kametani S (1988) Bacteria help desulfurize gas. Hydrocarb Process 76:76D–76FGoogle Scholar
  15. 15.
    van Scherpenzeel DA, Boon M, Ras C, Hansford GS, Heijnen JJ (1998) Kinetics of ferrous iron oxidation by Leptospirillum Bacteria in continuous cultures. Biotechnol Prog 14(3):425–433CrossRefPubMedGoogle Scholar
  16. 16.
    Wanner O, Reichert P (1996) Mathematical modeling of mixed-culture biofilms. Biotechnol Bioeng 49:172–184CrossRefPubMedGoogle Scholar

Copyright information

© Society for Industrial Microbiology and Biotechnology 2015

Authors and Affiliations

  • Sirous Ebrahimi
    • 1
  • Neda Faraghi
    • 1
  • Maryam Hosseini
    • 1
  1. 1.Faculty of Chemical Engineering, Biotechnology Research CenterSahand University of TechnologyTabrizIran

Personalised recommendations