Organic acids from lignocellulose: Candida lignohabitans as a new microbial cell factory
- 502 Downloads
Biorefinery applications require microbial cell factories for the conversion of various sugars derived from lignocellulosic material into value-added chemicals. Here, the capabilities of the yeast Candida lignohabitans to utilize a range of such sugars is characterized. Substrates efficiently converted by this yeast include the pentoses xylose and arabinose. Genetic engineering of C. lignohabitans with the isolated endogenous GAP promoter and GAP terminator was successful. GFP expression was used as a proof of functionality for the isolated transcription elements. Expression of lactate dehydrogenase and cis-aconitate decarboxylase resulted in stable and reproducible production of lactic acid and itaconic acid, respectively. The desired organic acids were accumulated converting pure sugars as well as lignocellulosic hydrolysates. C. lignohabitans proved therefore to be a promising reliable microbial host for production of organic acids from lignocellulosic material.
KeywordsMicrobial cell factory Candida lignohabitans Lactic acid production Itaconic acid production Biorefinery
The authors thank Verena Puxbaum for the help with the fluorescent microscopy pictures, Basso LR et al. for providing plasmids with C. albicans hygromycin B resistance gene, Rupert Koeberl (TDZ Ennstal, Reichraming, Austria) for the supply of pretreated lignocellulosic material and Novozymes (Bagsvaerd, Denmark) for samples of Cellic CTec2®. Finally, we thank the University of Natural Resources and Life Sciences Vienna—VIBT Imaging Center for access and expertise with the Leica DMI 6000 fluorescence microscope. This project was financed by the Austrian Research Promotion Agency (FFG), program “Intelligente Produktion” (FFG project n° 834297).
Conflict of interest
The authors declare no conflict of interest.
- 3.Bettiga M, Hahn-Hägerdal B, Gorwa-Grauslund MF (2008) Comparing the xylose reductase/xylitol dehydrogenase and xylose isomerase pathways in arabinose and xylose fermenting Saccharomyces cerevisiae strains. Biotechnol Biofuels 1:16. doi: 10.1186/1754-6834-1-16 CrossRefPubMedCentralPubMedGoogle Scholar
- 16.Kurtzman CP, Fell JW, Boekhout T (2011) The Yeasts, a taxonomic study, 5th edn. Elsevier Science B.V, AmsterdamGoogle Scholar
- 17.Limtong S, Sumpradit T, Kitpreechavanich V et al (2000) Effect of acetic acid on growth and ethanol fermentation of xylose fermenting yeast and Saccharomyces cerevisiae. Kasetsart J Nat Sci 34:64–73Google Scholar
- 25.Sambrook J, Russell D (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, NYGoogle Scholar