Habitat-specific type I polyketide synthases in soils and street sediments
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Actinomycetes produce many pharmaceutically useful compounds through type I polyketide biosynthetic pathways. Soil has traditionally been an important source for these actinomycete-derived pharmaceuticals. As the rate of antibiotic discovery has decreased and the incidence of antibiotic resistance has increased, researchers have looked for alternatives to soil for bioprospecting. Street sediment, where actinomycetes make up a larger fraction of the bacterial population than in soil, is one such alternative environment. To determine if these differences in actinomycetal community structure are reflected in type I polyketide synthases (PKSI) distribution, environmental DNA from soils and street sediments was characterized by sequencing amplicons of PKSI-specific PCR primers. Amplicons covered two domains: the last 80 amino acids of the ketosynthase (KS) domain and the first 240 amino acids of the acyltransferase (AT) domain. One hundred and ninety clones from ten contrasting soils from six regions and nine street sediments from six cities were sequenced. Twenty-five clones from two earthworm-affected samples were also sequenced. UniFrac lineage-specific analysis identified two clades that clustered with actinomycetal GenBank matches that were street sediment-specific, one similar to the PKSI segment of the mycobactin siderophore involved in mycobacterial virulence. A clade of soil-specific sequences clustered with GenBank matches from the ambruticin and jerangolid pathways of Sorangium cellulosum. All three of these clades were found in sites >700 km apart. Street sediments are enriched in actinomycetal PKSIs. Non-actinomycetal PKSI pathways may be more chemically diverse than actinomycetal PKSIs. Common soil and street sediment PKIs are globally distributed.
KeywordsNatural product discovery Polyketides Urban microbiology Bioprospecting Mycobactin
Dr. Meng Wang was kind enough to translate the paper “Screening identifying and functional analysis of polyketide synthase I domains from soil and seawater of Yangshan Harbour” into English for us. We thank Dr. A. Poulain for providing the sample from Resolute Bay, Canada, Dr. Rashid Nazir for providing the Faisalabad Clock tower sample, Dr. Declan Hill for providing the street sample from Budapest, Hungary, and Keavin M. Stanford-Finnerty for the beach sand sample from Prince Edward Island, Canada. Our laboratory work was helped by Jean Bjornson of the Geography Dept of the University of Ottawa who assisted with soil analysis and Daniël van Middelkoop and Sarina de Roos who carried out cloning and sequencing as part of a work experience program for the School for Biomedical Laboratory Techniques in Groningen, The Netherlands. Funding for this work was provided by the NATO Programme Security Through Science ESP.EAP.CLG 981785, the Ministry of Education, Youth and Sports of the Czech Republic (LC06066, 2B06154), the Research Plan of the Institute of Soil Biology (AV 0Z 6066 0521), the Natural Sciences and Engineering Council of Canada, the Ministry of Research and Innovation, and the University of Ottawa.
- 3.Ayuso–Sacido A, Genilloud O (2005) New PCR primers for the screening of NRPS and PKS-I systems in actinomycetes: detection and distribution of these biosynthetic gene sequences in major taxonomic groups. Microb Ecol 49:24–49Google Scholar
- 6.Connon S A, Lester ED, Shafaat HS, Obenhuber DC, Ponce A (2007) Bacterial diversity in hyperarid Atacama Desert soils. J Geophys Res 112: G04S17, doi: 10.1029/2006JG000311
- 7.Dong XY, Wang LH, Sun MJ, Zong Y, Jiao YL, Jiao BH (2008) Screening, identifying and function analysis of polyketide synthase I domains from soil and seawater of Yangshan Harbor. Microbiology 35:1359–1366Google Scholar
- 9.Faizal I, Lestari R, Kurnia F, Latif A, Hadianto D, Kusumawati N, Rachmawati I, Marwoto B, Purbowasito W (2008) Polymorphism analysis of polyketide synthase gene from Actinomycetes genome DNA of Taman Nasional Gunung Halimun soil by using metagenome method. J Biotechnology Res Tropical Reg 1: biotechindonesia.org/journal/jbr/jbr-2008-00-01/jrb-1-08-2.pdfGoogle Scholar
- 12.Ginolhac A, Jarrin C, Gillet B, Robe P, Pujic P, Tuphile K, Bertrand H, Vogel TM, Perrière G, Simonet P, Nalin R (2004) Phylogenetic analysis of polyketide I domains from soil metagenomic libraries allows selection of promising clones. Appl Environ Microbiol 70:5522–5527PubMedCentralPubMedCrossRefGoogle Scholar
- 15.Haydock SF, Aparicio JF, Molnár I, Schwecke T, Khaw LE, König A, Marsden AFA, Galloway IS, Staunton J, Leadlay PF (1995) Divergent sequence motifs correlated with the substrate specificity of (methlyl) malonyl-CoA: acyl carrier protein during transcyclase domains in modular polyketide synthases. FEBS Lett 374:246–248PubMedCrossRefGoogle Scholar
- 24.Luo K, Du G-P, Zhao Z-X, Xie BY, Li D-J (2010) Phylogenetic analysis of type I polyketide synthase and non-ribosomal peptide synthase genes from Mila Mountain in Tibet plateau. J Hunan Agric Uni (Nat Sci) 36:506–511Google Scholar
- 28.Paradis A (2006) Analysis of phylogenetics and evolution. Springer, Berlin Heidelberg New YorkGoogle Scholar