Journal of Industrial Microbiology & Biotechnology

, Volume 40, Issue 11, pp 1231–1240 | Cite as

Pollen baiting facilitates the isolation of marine thraustochytrids with potential in omega-3 and biodiesel production

Bioenergy/Biofuels/Biochemicals

Abstract

Marine heterotrophic microbes are capable of accumulating large amounts of lipids, omega-3 fatty acids, carotenoids, and have potential for biodiesel production. Pollen baiting using Pinus radiata pollen grain along with direct plating techniques were used in this study as techniques for the isolation of oil-producing marine thraustochytrid species from Queenscliff, Victoria, Australia. Thirteen isolates were obtained using either direct plating or using pine pollen, with pine pollen acting as a specific substrate for the surface attachment of thraustochytrids. The isolates obtained from the pollen baiting technique showed a wide range of docosahexaenoic acid (DHA) accumulation, from 11 to 41 % of total fatty acid content (TFA). Direct plating isolates showed a moderate range of DHA accumulation, from 19 to 25 % of TFA. Seven isolates were identified on the basis of 18S rRNA sequencing technique as Thraustochytrium species, Schizochytrium species, and Ulkenia species. Although both methods appear to result in the isolation of similar strains, pollen baiting proved to be a simpler method for the isolation of these relatively slow-growing organisms.

Keywords

Thraustochytrium sp. Schizochytrium sp. Isolation Pollen grain Direct plating PUFAs 18S rRNA Biodiesel 

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Copyright information

© Society for Industrial Microbiology and Biotechnology 2013

Authors and Affiliations

  1. 1.Centre for Chemistry and BiotechnologyDeakin UniversityGeelongAustralia
  2. 2.Marine Biodiversity and Biosecurity, NIWAWellingtonNew Zealand
  3. 3.Centre for Chemistry and BiotechnologyDeakin UniversityGeelongAustralia

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