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Journal of Industrial Microbiology & Biotechnology

, Volume 40, Issue 11, pp 1263–1271 | Cite as

Scalable economic extracellular synthesis of CdS nanostructured particles by a non-pathogenic thermophile

  • Ji-Won Moon
  • Ilia N. Ivanov
  • Chad E. Duty
  • Lonnie J. Love
  • Adam J. Rondinone
  • Wei Wang
  • Yi-Liang Li
  • Andrew S. Madden
  • Jennifer J. Mosher
  • Michael Z. Hu
  • Anil K. Suresh
  • Claudia J. Rawn
  • Hyunsung Jung
  • Robert J. Lauf
  • Tommy J. Phelps
Fermentation, Cell Culture and Bioengineering

Abstract

We report microbially facilitated synthesis of cadmium sulfide (CdS) nanostructured particles (NP) using anaerobic, metal-reducing Thermoanaerobacter sp. The extracellular CdS crystallites were <10 nm in size with yields of ~3 g/L of growth medium/month with demonstrated reproducibility and scalability up to 24 L. During synthesis, Thermoanaerobacter cultures reduced thiosulfate and sulfite salts to H2S, which reacted with Cd2+ cations to produce thermodynamically favored NP in a single step at 65 °C with catalytic nucleation on the cell surfaces. Photoluminescence (PL) analysis of dry CdS NP revealed an exciton-dominated PL peak at 440 nm, having a narrow full width at half maximum of 10 nm. A PL spectrum of CdS NP produced by dissimilatory sulfur reducing bacteria was dominated by features associated with radiative exciton relaxation at the surface. High reproducibility of CdS NP PL features important for scale-up conditions was confirmed from test tubes to 24 L batches at a small fraction of the manufacturing cost associated with conventional inorganic NP production processes.

Keywords

CdS nanostructured particles Nano-biotechnology Thermoanaerobacter Fermentation Photoluminescence Scalable synthesis 

Notes

Acknowledgments

This research was supported by the Department of Energy’s (DOE) Advanced Manufacturing Office (AMO), Nanomanufacturing for Energy Efficiency (NT08845) and by the Laboratory Directed Research and Development Program of ORNL (L05512). Part of this research was conducted at the Center for Nanophase Materials Sciences, which is sponsored at the ORNL Scientific User Facilities Division, Office of Basic Energy Sciences, U.S. DOE. The ORNL is managed by UT-Battelle, LLC, for the U.S. DOE under contract DE-AC05-00OR22725. The authors also appreciate James G. Elkins for constructive comments, Tae Hwan Kim for peak analysis, and Sue Carroll for cell counting.

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Copyright information

© Society for Industrial Microbiology and Biotechnology (Outside the USA) 2013

Authors and Affiliations

  • Ji-Won Moon
    • 1
  • Ilia N. Ivanov
    • 2
  • Chad E. Duty
    • 3
  • Lonnie J. Love
    • 4
  • Adam J. Rondinone
    • 2
  • Wei Wang
    • 5
  • Yi-Liang Li
    • 6
  • Andrew S. Madden
    • 7
  • Jennifer J. Mosher
    • 8
  • Michael Z. Hu
    • 9
  • Anil K. Suresh
    • 1
    • 10
  • Claudia J. Rawn
    • 3
  • Hyunsung Jung
    • 1
  • Robert J. Lauf
    • 1
  • Tommy J. Phelps
    • 1
  1. 1.Biosciences DivisionOak Ridge National Laboratory (ORNL)Oak RidgeUSA
  2. 2.Center for Nanophase Materials SciencesORNLOak RidgeUSA
  3. 3.Materials Science and Technology DivisionORNLOak RidgeUSA
  4. 4.Measurement Science and Systems Engineering DivisionORNLOak RidgeUSA
  5. 5.Environmental Sciences DivisionORNLOak RidgeUSA
  6. 6.Department of Earth SciencesThe University of Hong KongHong KongChina
  7. 7.School of Geology and GeophysicsUniversity of OklahomaNormanUSA
  8. 8.Stroud Water Research CenterAvondaleUSA
  9. 9.Energy and Transportation Science DivisionORNLOak RidgeUSA
  10. 10.Department of Molecular MedicineCity of HopeDuarteUSA

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