Journal of Industrial Microbiology & Biotechnology

, Volume 40, Issue 10, pp 1083–1093 | Cite as

A novel neutral xylanase with high SDS resistance from Volvariella volvacea: characterization and its synergistic hydrolysis of wheat bran with acetyl xylan esterase

  • Fei Zheng
  • Jingxuan Huang
  • Yuhao Yin
  • Shaojun Ding
Biocatalysis

Abstract

A neutral xylanase (XynII) from Volvariella volvacea was identified and characterized. Unlike other modular xylanases, it consists of only a single GH10 catalytic domain with a unique C-terminal sequence (W-R-W-F) and a phenylalanine and proline-rich motif (T-P-F-P-P-F) at N-terminus, indicating that it is a novel GH10 xylanase. XynII exhibited optimal activity at pH 7 and 60 °C and stability over a broad range of pH 4.0–10.0. XynII displayed extreme highly SDS resistance retaining 101.98, 92.99, and 69.84 % activity at the presence of 300 mM SDS on birchwood, soluble oat spelt, and beechwood xylan, respectively. It remained largely intact after 24 h of incubation with proteinase K at a protease to protein ratio of 1:50 at 37 °C. The kinetic constants Km value towards beechwood xylan was 0.548 mg ml−1, and the kcat/Km ratio, reflecting the catalytic efficiency of the enzyme, was 126.42 ml mg−1 s−1 at 60 °C. XynII was a true endo-acting xylanase lacking cellulase activity. It has weak activity towards xylotriose but efficiently hydrolyzed xylans and xylooligosaccharides larger than xylotriose mainly to xylobiose. Synergistic action with acetyl xylan esterase (AXEI) from V. volvacea was observed for de-starched wheat bran. The highest degree of synergy (DS 1.42) was obtained in sequential reactions with AXEI digestion preceding XynII. The high SDS resistance and intrinsic stability suggested XynII may have potential applications in various industrial processes especially for the detergent and textile industries and animal feed industries.

Keywords

Volvariella volvacea Xylanase SDS resistance Synergistic action Acetyl xylan esterase 

Abbreviations

GH

Glycoside hydrolase family

PNP-glycoside

ρ-Nitrophenyl-glycosides

PNPG

ρ-Nitrophenyl-glucopyranoside

PNPX

ρ-Nitrophenyl-xylopyranoside

PNPA

ρ-Nitrophenyl-arabinofuranoside

CMC

Carboxymethylcellulose

XOS

Xylooligosaccharides

X1

Xylose

X2

Xylobiose

X3

Xylotriose

X4

Xylotetraose

X5

Xylopentaose

X6

Xylohexaose

EST

Expressed sequence tag

SDS

Sodium dodecyl sulfate

SDS-PAGE

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis

HPAEC-PAD

High-performance anion exchange chromatography with pulsed amperometric detection

Notes

Acknowledgments

This work was supported by a research grant (no. 31270628) from the National Natural Science Foundation of China, a Project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions, and the Doctorate Fellowship Foundation of Nanjing Forestry University.

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Copyright information

© Society for Industrial Microbiology and Biotechnology 2013

Authors and Affiliations

  • Fei Zheng
    • 1
  • Jingxuan Huang
    • 1
  • Yuhao Yin
    • 1
  • Shaojun Ding
    • 1
  1. 1.Department of Biological EngineeringNanjing Forestry UniversityNanjingChina

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