Journal of Industrial Microbiology & Biotechnology

, Volume 40, Issue 9, pp 995–1003 | Cite as

Rapid enrichment of (homo)acetogenic consortia from animal feces using a high mass-transfer gas-lift reactor fed with syngas

  • Shinyoung Park
  • Muhammad Yasin
  • Daehee Kim
  • Hee-Deung Park
  • Chang Min Kang
  • Duk Jin Kim
  • In Seop Chang
Biotechnology Methods


A gas-lift reactor having a high mass transfer coefficient (k L a = 80.28 h−1) for a relatively insoluble gas (carbon monoxide; CO) was used to enrich (homo)acetogens from animal feces. Samples of fecal matter from cow, rabbit, chicken, and goat were used as sources of inoculum for the enrichment of CO and H2 utilizing microbial consortia. To confirm the successful enrichment, the Hungate roll tube technique was employed to count and then isolate putative CO utilizers. The results of this work showed that CO and H2 utilizing consortia were established for each inoculum source after 8 days. The number of colony-forming units in cow, rabbit, chicken, and goat fecal samples were 3.83 × 109, 1.03 × 109, 8.3 × 108, and 3.25 × 108 cells/ml, respectively. Forty-two colonies from the animal fecal samples were screened for the ability to utilize CO/H2. Ten of these 42 colonies were capable of utilizing CO/H2. Five isolates from cow feces (samples 5, 6, 8, 16, and 22) were highly similar to previously unknown (homo)acetogen, while cow-7 has shown 99 % similarity with Acetobacterium sp. as acetogens. On the other hand, four isolates from chicken feces (samples 3, 8, 10, and 11) have also shown high CO/H2 utilizing activity. Hence, it is expected that this research could be used as the basis for the rapid enrichment of (homo)acetogenic consortia from various environmental sources.


Gas-lift reactor Mass transfer (Homo)acetogen Synthesis gas 



This work was supported by the Development of Biohydrogen Production Technology using the Hyperthermophilic Archaea program of the Ministry of Land, Transport, and Maritime Affairs, Republic of Korea. This work was also supported by the Research Program for Agricultural Science and Technology Development (project no. PJ008517032012) and by the Advanced Biomass R&D Center (ABC-2011-0031355) funded by the Ministry of Education, Science and Technology, and the Korea Environment Corporation.


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Copyright information

© Society for Industrial Microbiology and Biotechnology 2013

Authors and Affiliations

  • Shinyoung Park
    • 1
  • Muhammad Yasin
    • 1
  • Daehee Kim
    • 2
  • Hee-Deung Park
    • 3
  • Chang Min Kang
    • 4
  • Duk Jin Kim
    • 5
  • In Seop Chang
    • 1
  1. 1.School of Environmental Science and EngineeringGwangju Institute of Science and Technology (GIST)GwangjuRepublic of Korea
  2. 2.Jeju Global Research CenterKorea Institute of Energy ResearchJejuRepublic of Korea
  3. 3.School of Civil, Environmental and Architectural EngineeringKorea UniversitySeoulRepublic of Korea
  4. 4.Gyeongnam Department of Environmental Toxicology & ChemistryKorea Institute of ToxicologyJinjuRepublic of Korea
  5. 5.Department of Water and Sewage SupportKorea Environment CorporationIncheonRepublic of Korea

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