Detection of a novel active transposable element in Caldicellulosiruptor hydrothermalis and a new search for elements in this genus

  • Daehwan Chung
  • Joel Farkas
  • Janet Westpheling
Short Communication


We show that a previously annotated hypothetical protein is the transposase of a new and active IS element, ISCahy1, widespread in Caldicellulosiruptor species. Transposition generated an 11-bp direct repeat at the insertion site in Caldicellulosiruptor hydrothermalis, suggesting a cut-and-paste mechanism. The discovery of an active insertion sequence in Caldicellulosiruptor species led to a survey of potential IS elements in the genome sequences of eight Caldicellulosiruptor species that identified several new elements, including one novel to this genus.


Transposition Thermophilic anaerobes Caldicellulosiruptor IS element ISCahy1 ISCbe4 



We thank Lee Lynd, Dan Olson, and Adam Guss for sharing unpublished results, Jenna Oberstaller for assistance in genome sequence analysis, and Minseok Cha and Jennifer Copeland for helpful discussions during the course of the work. This work was supported by The BioEnergy Science Center supported by a U.S. Department of Energy Bioenergy Research Center supported by the Office of Biological and Environmental Research in the DOE Office of Science. JF was supported in part by a predoctoral Graduate Training In Genetics grant (NIH 5T32GM007103-30) to the Genetics Department of the University of Georgia.

Supplementary material

10295_2013_1244_MOESM1_ESM.docx (21 kb)
Supplementary material 1 (DOCX 20 kb)


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Copyright information

© Society for Industrial Microbiology and Biotechnology 2013

Authors and Affiliations

  • Daehwan Chung
    • 1
    • 2
  • Joel Farkas
    • 1
    • 2
  • Janet Westpheling
    • 1
    • 2
  1. 1.Department of GeneticsUniversity of GeorgiaAthensUSA
  2. 2.The BioEnergy Science Center, Department of EnergyOak Ridge National LaboratoryOak RidgeUSA

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